Human NF-L Antibody (1033901)

R&D Systems | Catalog # MAB10680

R&D Systems

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Dual RNAscope ISH-IHC Compatible, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1033901
View all NF-L Primary Antibodies »

Product Specifications

Immunogen

Human  NF-L peptide

Specificity

Detects human NF-L in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NF-L Antibody (1033901)

NF‑L in Human Brain (Hippocampus).

NF‑L was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human NF‑L Monoclonal Antibody (Catalog # MAB10680) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

NF-L in U251 human myeloma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative).

NF‑L was detected in immersion fixed U251 human myeloma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative) using Mouse Anti-Human NF‑L Monoclonal Antibody (Catalog # MAB10680) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Detection of NEFL in Human Cerebellum Using Dual RNAscope® ISH and IHC.

NEFL mRNA (red) and protein (green/blue) was detected in formalin-fixed paraffin-embedded tissue sections of Human Cerebellum. ACD’s Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-NEFL (Catalog# 468671) and Mouse Anti-Human NEFL monoclonal antibody (R&D Systems, Catalog# MAB10680) at 10 ug/mL. Tissue was stained using RNAscope® 2.5 HD Detection Reagents-RED (Catalog# 322360), Mouse IgG VisUCyte HRP Polymer Antibody (VC001), and RNAscope® 2.5 LS Green Accessory Pack (Catalog# 322550). Tissue was counterstained with 50% hematoxylin (purple).

Applications for Human NF-L Antibody (1033901)

Application
Recommended Usage

Dual RNAscope ISH-IHC Compatible

5-15 µg/mL
Sample: Formalin-fixed paraffin-embedded tissue sections of Human Cerebellum. 

Immunocytochemistry

8 µg/mL
Sample: Immersion fixed U251 human myeloma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative)

Immunohistochemistry

8-25 µg/mL
Sample: Human brain (hippocampus)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: NF-L

NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Entrez Gene IDs

4747 (Human)

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for Human NF-L Antibody (1033901)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NF-L Antibody (1033901)

For research use only

Customer Reviews for Human NF-L Antibody (1033901)

There are currently no reviews for this product. Be the first to review Human NF-L Antibody (1033901) and earn rewards!

Have you used Human NF-L Antibody (1033901)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies