Human Nogo-C Antibody (499825)

R&D Systems | Catalog # MAB5766

R&D Systems
Discontinued Product
MAB5766 has been discontinued. An alternative/replacement product is available: AF5766. View all Nogo-C products.

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 499825
View all Nogo-C Primary Antibodies »

Product Specifications

Immunogen

Human Nogo-C synthetic peptide
MDGQKKNWKDKVVD
Accession # NP_008939

Specificity

Detects human Nogo-C in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Nogo-A or rhNogo-B is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Applications for Human Nogo-C Antibody (499825)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Nogo-C

Nogo belongs to the reticulon family of transmembrane proteins, which lack an N-terminal signal sequence and possess a 200 amino acid (aa) C-terminus that contains two transmembrane domains with an ER-retention motif. They show punctate intracellular distribution within the ER that is reminescent of a reticulum. The five Nogo isoforms vary from 199 aa (Nogo-C) to 1192 aa (Nogo-A; also called reticulon-4) in humans. Nogo-B or -C may form complexes with Nogo-A, and all may be expressed in neurons, endothelial cells, oligodendrocytes, fibroblasts and myoblasts. Of 11 aa unique to the N-terminus of human Nogo-C, 9 and 10 aa are identical in mouse and rat Nogo-C, respectively.

Long Name

Reticulon 4C

Alternate Names

NogoC, RTN4C

Entrez Gene IDs

57142 (Human); 68585 (Mouse); 83765 (Rat)

Gene Symbol

RTN4

UniProt

NP_008939

Additional Nogo-C Products

Product Documents for Human Nogo-C Antibody (499825)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Nogo-C Antibody (499825)

For research use only

Customer Reviews for Human Nogo-C Antibody (499825)

There are currently no reviews for this product. Be the first to review Human Nogo-C Antibody (499825) and earn rewards!

Have you used Human Nogo-C Antibody (499825)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies