|Detection of Human Nogo‑C by Western Blot. Western blot shows lysates of human skeletal muscle. PVDF Membrane was probed with 1 µg/mL of Human Nogo‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5766) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nogo‑C at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Nogo‑C in Human Brain. Nogo‑C was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Human Nogo‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5766) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
Nogo belongs to the reticulon family of transmembrane proteins, which lack an N-terminal signal sequence and possess a 200 amino acid (aa) C-terminus that contains two transmembrane domains with an ER-retention motif. They show punctate intracellular distribution within the ER that is reminescent of a reticulum. The five Nogo isoforms vary from 199 (Nogo-C) to 1192 (Nogo-A; also called reticulon-4) aa in humans. Nogo-B or -C may form complexes with Nogo-A, and all may be expressed in neurons, endothelial cells, oligodendrocytes, fibroblasts and myoblasts. Of 11 aa unique to the N-terminus of human Nogo-C, 9 and 10 aa are identical in mouse and rat Nogo-C, respectively.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided