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Human Pax3/Pax7 Antibody

Catalog #: AF2457
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2457
AF2457-SP

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Detection of Pax3/Pax7 in Human Skin.
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Citations (5)
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Human Pax3/Pax7 Antibody Summary

Species Reactivity
Human
Specificity
Detects Pax3/Pax7 in direct ELISAs and Western blots. In Western blots, approximately 2% cross-reactivity with recombinant human (rh) Pax5, under reducing conditions, is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Pax3
Met1-Ser215
Accession # NP_000429
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Pax3
Immunohistochemistry
3 µg/mL
Immersion fixed paraffin-embedded sections of human skin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Detection of Pax3/Pax7 in Human Skin. View Larger

Detection of Pax3/Pax7 in Human Skin. Pax3 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Pax3/Pax7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2457) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus of melanocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pax3/Pax7

Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. There is 98% homology between human and mouse Pax3.

Long Name
Paired Box Gene 3 /Paired Box Gene 7
Alternate Names
Pax3/Pax7

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Citations for Human Pax3/Pax7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. In�vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion
    Authors: S Gupta, R Kawaguchi, E Heinrichs, S Gallardo, S Castellano, I Mandric, BG Novitch, SJ Butler
    Oncogene, 2022-07-19;40(3):111119.
    Species: Human
    Sample Types: Embryoid Bodies
    Applications: IHC
  2. Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells
    Authors: Sandeep Gupta, Ken Yamauchi, Bennett G. Novitch, Samantha J. Butler
    STAR Protocols
  3. Attenuated Fgf Signaling Underlies the Forelimb Heterochrony in the Emu Dromaius novaehollandiae
    Authors: John J. Young, Phil Grayson, Scott V. Edwards, Clifford J. Tabin
    Current Biology
  4. Inhibitory Smads differentially regulate cell fate specification and axon dynamics in the dorsal spinal cord.
    Authors: Hazen VM, Phan KD, Hudiburgh S, Butler SJ
    Dev. Biol., 2011-06-22;356(2):566-75.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  5. A highly enriched niche of precursor cells with neuronal and glial potential within the hair follicle dermal papilla of adult skin.
    Authors: Hunt DP, Morris PN, Sterling J, Anderson JA, Joannides A, Jahoda C, Compston A, Chandran S
    Stem Cells, 2007-09-27;26(1):163-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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