Human phospho-p53 (S392) Antibody (2185A)

R&D Systems | Catalog # MAB2996

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 2185A
View all p53 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containng the human p53 S392 site
Accession # P04637

Specificity

Detects human p53 when phoshorylated at S392

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human phospho-p53 (S392) Antibody (2185A)

Detection of Human Phospho-p53 (S392) antibody by Western Blot.

Detection of Human Phospho-p53 (S392) by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1uM Camptothecin (CPT) for 5 hours. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-p53 (S392) Monoclonal Antibody (Catalog # MAB2996) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-p53 (S392) at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-p53 (S392) antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

Phospho-p53 (S392) in MCF‑7 Human Cell Line.

p53 phosphorylated at S392 was detected in immersion fixed MCF-7 human breast cancer cell line using Rabbit Anti-Human Phospho-p53 (S392) Monoclonal Antibody (Catalog # MAB2996) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human phospho-p53 (S392) Antibody (2185A)

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Western Blot

2 µg/mL
Sample: MCF‑7 human breast cancer cell line

Reviewed Applications

Read 1 review rated 5 using MAB2996 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p53

The p53 tumor suppressor protein acts to enforce cell cycle checkpoints or signal apoptosis in cells that have incurred genotoxic damage. The ATM or ATR kinases can phosphorylate p53 at serine 15 (S15), which leads to cell cycle arrest. Serine 15 phosphorylation leads to p53 stabilization and enhances transactivation of p53 target genes.

Alternate Names

BCC7, LFS1, TP53, TRP53

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for Human phospho-p53 (S392) Antibody (2185A)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human phospho-p53 (S392) Antibody (2185A)

For research use only

Customer Reviews for Human phospho-p53 (S392) Antibody (2185A) (1)

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  • Human Phospho-p53 (S392) Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: U937 human histiocytic lymphoma cell line
    Species: Human
    Verified Customer | Posted 04/05/2022
    Human phospho-p53 (S392) Antibody (2185A) MAB2996

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Protocols

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