Human RIPK3/RIP3 Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB7604U Datasheet / COA / SDS

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FAB7604U-100UG

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All RIPK3/RIP3 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RIPK3/RIP3 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human RIPK3/RIP3 in direct ELISAs.

Source

Monoclonal Mouse IgG1 Clone # 780115

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human RIPK3/RIP3
Met1-Arg218
Accession # Q9Y572

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: RIPK3/RIP3

RIP3 (Receptor-Interacting Protein 3), also known as RIPK3, is a 518 amino acid (aa), 60 kDa phosphoprotein that contains an N-terminal protein kinase domain and a C-terminal interaction motif with which it binds RIP1. It converts cell response to TNF-a from apoptosis (RIP1 via Fas) to programmed necrosis (RIP1/RIP3) by inducing reactive oxygen species production. Human RIP3 (aa 1-218) shares approximately 74% aa sequence identity with mouse and rat RIP3. Alternately spliced human b (252 aa) and g (231 aa) isoforms diverge after aa 219 and 221, respectively, and are found to antagonize RIP3.

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 3

Entrez Gene IDs

11035 (Human); 56532 (Mouse); 246240 (Rat)

Alternate Names

EC 2.7.11.1; Receptor Interacting Serine/Threonine Kinase 3; Receptor-interacting protein 3; receptor-interacting serine/threonine-protein kinase 3; receptor-interacting serine-threonine kinase 3; RIP3; RIP-3; RIPK3; RIP-like protein kinase 3