Human RIPK3/RIP3 Antibody (780115)

R&D Systems | Catalog # MAB7604

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Porcine

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Co-Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 780115
View all RIPK3/RIP3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RIPK3/RIP3
Met1-Arg218
Accession # Q9Y572

Specificity

Detects human RIPK3/RIP3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human RIPK3/RIP3 Antibody (780115)

Detection of Human RIPK3/RIP3 antibody by Western Blot.

Detection of Human RIPK3/RIP3 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB7604) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RIPK3/RIP3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RIPK3/RIP3 antibody in Human Spleen by Immunohistochemistry (IHC-P).

RIPK3/RIP3 in Human Spleen.

RIPK3/RIP3 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB7604) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of RIPK3/RIP3 by Western Blot

Detection of RIPK3/RIP3 by Western Blot

Cell death signaling analysis in PeV-A3-infected GBM cells. (A) GBM cells were infected with PeV-A3 at MOI = 1 for 6, 24, and 48 h. Cell extracts were employed to analyze cell death signaling pathways by Western blotting using anti-PeV VP0, anti-caspase 3, anti-PARP, anti-caspase 8, anti-caspase 9, anti-FASLG, anti-CD40, anti-LC3, anti-p62, anti-beclin 1, anti-phospho-NF-kappa B p65 (Ser536), anti-NLRP3, anti-IL1 beta, anti-RIPK1, anti-RIPK3, anti-GSDMD, and anti-beta -actin. (B) Quantified expression of different proteins was normalized to beta -actin. Data are mean ± SD of three independent experiments. Student’s t-test, *p < 0.05; **p < 0.01 compared with mock controls. PeV-A3, parechovirus type 3; GBM, glioblastoma; MOI, multiplicity of infection. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34899705), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human RIPK3/RIP3 Antibody (780115)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and HT‑29 human colon adenocarcinoma cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB7604 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RIPK3/RIP3

RIP3 (Receptor-Interacting Protein 3), also known as RIPK3, is a 518 amino acid (aa), 60 kDa phosphoprotein that contains an N-terminal protein kinase domain and a C-terminal interaction motif with which it binds RIP1. It converts cell response to TNF-a from apoptosis (RIP1 via Fas) to programmed necrosis (RIP1/RIP3) by inducing reactive oxygen species production. Human RIP3 (aa 1-218) shares approximately 74% aa sequence identity with mouse and rat RIP3. Alternately spliced human b (252 aa) and g (231 aa) isoforms diverge after aa 219 and 221, respectively, and are found to antagonize RIP3.

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 3

Alternate Names

RIP3

Entrez Gene IDs

11035 (Human); 56532 (Mouse); 246240 (Rat)

Gene Symbol

RIPK3

UniProt

Q9Y572 (Human)

Additional RIPK3/RIP3 Products

Product Documents for Human RIPK3/RIP3 Antibody (780115)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human RIPK3/RIP3 Antibody (780115)

For research use only

Related Research Areas

Apoptosis Adaptor Proteins
NFkB Pathway

Citations for Human RIPK3/RIP3 Antibody (780115)

Customer Reviews for Human RIPK3/RIP3 Antibody (780115) (2)

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2 Customer Ratings
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Customer Images

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  • Human RIPK3/RIP3 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Nervous tissue
    Species: Human
    Verified Customer | Posted 10/18/2021
    Human RIPK3/RIP3 Antibody (780115) MAB7604
  • Human RIPK3/RIP3 Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: HT-29 colon cancer cell line
    Species: Human
    Verified Customer | Posted 10/16/2020
    Total RIPK3
    Human RIPK3/RIP3 Antibody (780115) MAB7604
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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