Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Avian - Chicken

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ROBO2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ROBO2
Ser22-Ala312, RPVA, Pro313-Pro859
Accession # Q9HCK4

Specificity

Detects human ROBO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO3 and rhROBO4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human ROBO2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: NTera‑2 human testicular embryonic carcinoma cell line differentiated with retinoic acid

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human neural progenitor cells differentiated by growth factor withdrawal

Western Blot

0.1 µg/mL
Sample: Recombinant Human ROBO2 Fc Chimera (Catalog # 3147-RB)

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ROBO2

Human ROBO2 is a 175 kDa member of the ROBO/roundabout receptor family and is a 1357 amino acid (aa) type I transmembrane (TM) protein. It contains an extracellular domain (ECD) comprised of five C2-type Ig-like domains and three fibronectin type III domains. ROBO receptors interact with SLIT proteins to regulate axon guidance. Similar to ROBO1, the first two Ig-like domains of ROBO2 are predicted to be important for SLIT binding. Alternate splice forms of ROBO2 exist. The ROBO2 ECD in human shares 98% aa identity with the ROBO2 ECD in mouse and canine.

Long Name

Roundabout Homolog 2

Alternate Names

SAX3

Entrez Gene IDs

6092 (Human); 268902 (Mouse); 84409 (Rat)

Gene Symbol

ROBO2

UniProt

Q9HCK4

Additional ROBO2 Products

Product Documents for Human ROBO2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ROBO2 Antibody

For research use only

Citations for Human ROBO2 Antibody

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Protocols

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FAQs

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