Human RUNX3/CBFA3 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF3765G Datasheet / COA / SDS

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AF3765G-100UG

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All RUNX3/CBFA3 Antibodies

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Human RUNX3/CBFA3 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human RUNX3/CBFA3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows less than 1% cross‑reactivity with recombinant human (rh) CBFA1 and rhCBFA2.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RUNX3/CBFA3
Lys186-Tyr415
Accession # Q13761

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: RUNX3/CBFA3

RUNX3, also known as CBFA3 and PEBP2 alpha (polyomavirus enhancer binding protein 2 alpha ) is a member of the runt-related (RUNX) family of transcription factors. It is the DNA binding subunit of the heterodimeric transcription factor CBF/PEBP2. RUNX3 is a nuclear effector of the BMP/TGF-beta pathways and its inactivation or reduced expression is associated with numerous human malignancies. Two isoforms that differ in their N-terminal 5/19 residues have been reported. The 415 amino acid residue of human RUNX3 shares 91% amino acid sequence homology with mouse or rat RUNX3.

Long Name

Runt-related Transcription Factor 3

Entrez Gene IDs

864 (Human); 12399 (Mouse); 156726 (Rat)

Alternate Names

Acute myeloid leukemia 2 protein; AML2; AML2SL3/AKV core-binding factor alpha C subunit; CBFA3; CBFA3MGC16070; CBF-alpha-3; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2A3; PEBP2A3FLJ34510; PEBP2AC; PEBP2-alpha C; runt domain, alpha subunit 3; runt-related transcription factor 3; RUNX3; SL3-3 enhancer factor 1 alpha C subunit; transcription factor AML2