Human RUNX3/CBFA3 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF3765R Datasheet / COA / SDS

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AF3765R-100UG

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All RUNX3/CBFA3 Antibodies

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Human RUNX3/CBFA3 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human RUNX3/CBFA3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows less than 1% cross‑reactivity with recombinant human (rh) CBFA1 and rhCBFA2.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RUNX3/CBFA3
Lys186-Tyr415
Accession # Q13761

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: RUNX3/CBFA3

RUNX3, also known as CBFA3 and PEBP2 alpha (polyomavirus enhancer binding protein 2 alpha ) is a member of the runt-related (RUNX) family of transcription factors. It is the DNA binding subunit of the heterodimeric transcription factor CBF/PEBP2. RUNX3 is a nuclear effector of the BMP/TGF-beta pathways and its inactivation or reduced expression is associated with numerous human malignancies. Two isoforms that differ in their N-terminal 5/19 residues have been reported. The 415 amino acid residue of human RUNX3 shares 91% amino acid sequence homology with mouse or rat RUNX3.

Long Name

Runt-related Transcription Factor 3

Entrez Gene IDs

864 (Human); 12399 (Mouse); 156726 (Rat)

Alternate Names

Acute myeloid leukemia 2 protein; AML2; AML2SL3/AKV core-binding factor alpha C subunit; CBFA3; CBFA3MGC16070; CBF-alpha-3; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2A3; PEBP2A3FLJ34510; PEBP2AC; PEBP2-alpha C; runt domain, alpha subunit 3; runt-related transcription factor 3; RUNX3; SL3-3 enhancer factor 1 alpha C subunit; transcription factor AML2