Human S100A8 Antibody (749916)

R&D Systems | Catalog # MAB4570

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 749916
View all S100A8 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100A8
Met1-Glu93
Accession # P05109

Specificity

Detects human S100A8 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) S100A8/A9 is observed and no cross-reactivity with rhS100A9, recombinant mouse (rm) S100A8, rmS100A9, or rmS100A8/A9 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human S100A8 Antibody (749916)

Detection of Human S100A8 antibody by Western Blot.

Detection of Human S100A8 by Western Blot.

Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100A8 Monoclonal Antibody (Catalog # MAB4570) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for S100A8 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A8 antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P).

S100A8 in Human Lung Cancer Tissue.

S100A8 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human S100A8 Monoclonal Antibody (Catalog # MAB4570) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human S100A8 by Western Blot

Detection of Human S100A8 by Western Blot

Quantitative PCR and immunoblotting of five candidate gene products in DLBCL and LCL lines. (A) Reverse transcriptase and real-time PCR analysis of mRNAs for BMP8A, CCR6, HOXA9, NANOG and S100A8 in LCL and DLBCL (HBL-2, HT, SU-DHL-5, U2932 and U2940) cell lines. Data were normalized by the amount of GAPDH mRNA, expressed relative to the corresponding value for LCL, and are shown as means ± SD from triplicate data. (B) After quantification of Western blot intensity, the relative expression levels of each protein are shown with normalization of GAPDH. U2940 cells have the highest expression of the stem cell genes, HOXA9 and NANOG. Molecular weight listed in parenthesis. Error bars represent the standard error of the mean of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, Student t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33288848), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human S100A8 by Western Blot

Detection of Human S100A8 by Western Blot

Quantitative PCR and immunoblotting of five candidate gene products in DLBCL and LCL lines. (A) Reverse transcriptase and real-time PCR analysis of mRNAs for BMP8A, CCR6, HOXA9, NANOG and S100A8 in LCL and DLBCL (HBL-2, HT, SU-DHL-5, U2932 and U2940) cell lines. Data were normalized by the amount of GAPDH mRNA, expressed relative to the corresponding value for LCL, and are shown as means ± SD from triplicate data. (B) After quantification of Western blot intensity, the relative expression levels of each protein are shown with normalization of GAPDH. U2940 cells have the highest expression of the stem cell genes, HOXA9 and NANOG. Molecular weight listed in parenthesis. Error bars represent the standard error of the mean of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, Student t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33288848), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human S100A8 Antibody (749916)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue

Western Blot

2 µg/mL
Sample: HL‑60 human acute promyelocytic leukemia cell line

Reviewed Applications

Read 1 review rated 5 using MAB4570 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A8

S100A8 (also known as MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca‑dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12-47 and 46-81) and one high-affinity Ca‑binding site (aa 59-70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP‑2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Long Name

S100 Calcium Binding Protein A8

Alternate Names

Calgranulin A, CFAG, MRP8

Entrez Gene IDs

6279 (Human); 20201 (Mouse)

Gene Symbol

S100A8

UniProt

P05109

Additional S100A8 Products

Product Documents for Human S100A8 Antibody (749916)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S100A8 Antibody (749916)

For research use only

Citations for Human S100A8 Antibody (749916)

Customer Reviews for Human S100A8 Antibody (749916) (1)

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  • Human S100A8 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Appendix
    Species: Human
    Verified Customer | Posted 10/25/2021
    Human S100A8 Antibody (749916) MAB4570

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