Serpin A4 is a member of the Serpin superfamily of the serine protease inhibitors (1). Known also as Kallistatin or proteinase inhibitor 4, Serpin A4 has been shown to function as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth (2, 3).
Human Serpin A4/Kallistatin Antibody [Biotin]
R&D Systems | Catalog # BAF1669
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A4/Kallistatin
Gln21-Pro427
Accession # AAA59454
Gln21-Pro427
Accession # AAA59454
Specificity
Detects human Serpin A4/Kallistatin in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross‑reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A5, rhSerpin C1, rhSerpin D1, rhSerpin E1, rhSerpin E2, and rhSerpin F2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Serpin A4/Kallistatin Antibody [Biotin]
Human Serpin A4 / Kallistatin ELISA Standard Curve
Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI) was serially diluted and captured by Mouse Anti-Human Serpin A4/Kallistatin Monoclonal Antibody (Catalog # MAB16691) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Serpin A4/Kallistatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1669) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human Serpin A4/Kallistatin Antibody [Biotin]
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI)
Sample: Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI)
Human Serpin A4/Kallistatin Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Serpin A4/Kallistatin
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Chen, V.C. et al. (2000) J. Biol. Chem. 275:40371.
- Miao, R.Q. et al. (2002) Blood 100:3245.
Alternate Names
Kallistatin
Entrez Gene IDs
5267 (Human)
Gene Symbol
SERPINA4
UniProt
Additional Serpin A4/Kallistatin Products
Product Documents for Human Serpin A4/Kallistatin Antibody [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Serpin A4/Kallistatin Antibody [Biotin]
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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