Human Serum Albumin Antibody (3B8-H3-G1) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-52357

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # 3B8-H3-G1

Format

Azide and BSA Free
View all Human Serum Albumin Primary Antibodies »

Product Specifications

Immunogen

Human Serum Albumin

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone 3B8-H3-G1 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone 3B8-H3-G1 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.

Scientific Data Images

Immunocytochemistry/ Immunofluorescence: Human Serum Albumin Antibody (3B8-H3-G1) - Chimeric - Azide and BSA Free [NBP3-52357]

Immunocytochemistry/ Immunofluorescence: Human Serum Albumin Antibody (3B8-H3-G1) - Chimeric - Azide and BSA Free [NBP3-52357]

Immunofluorescent staining of HepG2 cells using NBP3-52356 (the BSA Free version of this antibody) (green). DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the endogenous Human Serum Albumin protein in the cytoplasm of HepG2 cells.
Western Blot: Human Serum Albumin Antibody (3B8-H3-G1) - Chimeric - Azide and BSA Free [NBP3-52357]

Western Blot: Human Serum Albumin Antibody (3B8-H3-G1) - Chimeric - Azide and BSA Free [NBP3-52357]

Western blotting of 2 μL human serum with NBP3-52356 (the BSA Free version of this antibody), detected with anti-chicken-HRP.This antibody recognizes endogenous albumin protein in human serum at ~66 kDa.

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Western Blot

1:1000-1:3000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Human Serum Albumin

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Alternate Names

albumin, cell growth inhibiting protein 42, DKFZp779N1935, growth-inhibiting protein 20, PRO0883, PRO0903, PRO1341, serum albumin

Gene Symbol

ALB

Additional Human Serum Albumin Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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