Human Smad8 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF2309X Datasheet / COA / SDS

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AF2309X-100UG

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Human Smad8 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Smad8 in direct ELISAs and Western blots. In direct ELISAs, approximately 90% cross-reactivity with recombinant (rh) Smad1 is observed, approximately 20% cross-reactivity with rhSmad4 is observed, and less than 1% cross-reactivity with rhSma

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Smad8
Ala158-Ser233
Accession # NP_005896

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Smad8

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. Smad8 was originally described as MADH6 and is also known as Smad9 and MADH9. Upon signaling by some BMP family members, Smad8 is phosphorylated resulting in its association with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity.

Long Name

Mothers Against DPP Homolog 8

Entrez Gene IDs

4093 (Human)

Alternate Names

MADH6;MADH9;Mothers against decapentaplegic homolog;Mothers against decapentaplegic homolog 9;PPH2;SMAD8;SMAD8/9;SMAD8A;SMAD8B;SMAD9; Smad8; Smad9