Human SOX7 Antibody

Detection of SOX7 by Immunohistochemistry

Time‐course morphological analysis of Sox7 endothelial loss‐of‐function embryosA–X(A–C, G–I, M–O, S–U) Morphological characterization of the cardiac defects present in Sox7iECKO hearts compared to control hearts at E11.5 (A–C), E12.5 (G–I), E13.5 (M–O), and E16.5 (S–U) after Cre induction by tamoxifen injection at E9.5 and E10.5, by immunofluorescence with tissue‐specific markers CD31 (endocardium/endothelium; cyan), MF20 (myocardium; magenta), and nuclei (Hoechst; blue). (A, G, M, S) Low‐magnification images showing the general heart morphology. (B, C, H, I, N, O, T, U) High‐magnification images showing the morphology of the compact (arrows) and trabecular (arrowheads) myocardium, and coronary arteries (empty arrow) in the right (RV; B, H, N, T) and left (LV; C, I, O, U) ventricles. White contours in high‐magnification panels highlight the compact myocardium area. Morphological defects are evident from E13.5 (M–O) and maintained at E16.5 (S–U). (D–F, J–L, P–R, V–X) Quantification of total (D, J, P, V), compact and trabecular myocardium (E, F, K, L, Q, R, W, X) area in right (E, K, Q, W) and left (F, L, R, X) ventricular chambers of Sox7iECKO hearts compared to control hearts. Quantification results confirmed no significant difference in tissue area at E11.5 (D–F) and E12.5 (J–L). It also identified a significant increase in total and trabecular myocardium area at E13.5 (P–R) and the maintenance of the E14.5 cardiac defects at E16.5 (V–X). Biological replicates as per the number of data points in the graphs. Mean ± SEM; t‐test. P < 0.05 (*); P < 0.005 (**); P < 0.0005 (***); ns = not significant. Scale bars = 100 μm (A–C), 150 μm (G–I), 200 μm (M–O), and 300 μm (S–U). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37551717), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SOX7 by Immunohistochemistry

SOX7/17 expression depends on MEK1/2 signalling in the developing testis. Section or wholemount immunofluorescent imaging of E12.5 testes cultured for 24 or 72h with DMSO or 500nM of MEKi. A and C Section IF of cultured testes stained with DAPI (blue), SOX7/17 (red) and CD31 (endothelial cells and germ cells; cyan). B Three-dimensional stacked Z-series images of cultured testes showing Oct4-eGFP (germ cells; green), SOX7/17 (blue) and pERK1/2 (red). The DMSO and MEKi images should be viewed in conjunction with Movies S5-8 which reveal the 3D organisation of the cells in the tissue. Scale bar represents 100 μm. Arrows indicate SOX7/17 positive endothelial cells. Biological replicates; n = 3–4 per treatment Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39354506), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SOX7 by Immunohistochemistry

Specification of the coronary artery and dorsal aorta is dependent on endothelial Sox7 functionAWhole‐mount immunostaining of control and Sox7iECKO hearts at E14.5 after Cre induction by tamoxifen injection at E9.5, E10.5. Coronary vessels are stained with VEGFR2 (green), coronary arteries (arrows) and trabecular myocardium (TM) are both stained with CX40 (red). Asterisks show where the major distal coronary arteries are lost in the Sox7iECKO heart. The number of embryos showing the illustrated phenotype among the total examined is indicated.BWhole‐mount immunostaining of control and Sox7iECKO hearts at E13.5 after Cre induction at E9.5 and E10.5. Coronary vessels are stained with VEGFR2 (white) and endothelial nuclei with ERG (red). The coronary plexus front is outlined by the dotted lines.C–EGraphs showing quantification of coronary vessel density (C), ERG‐positive endothelial cells (D), and % of H3+ proliferative endothelial cells (E) in the coronary plexus. Scored sibling control, n = 4; Sox7iECKO mutant embryos, n = 4 embryos; Mean ± SEM; Mann–Whitney U‐test. ns = not significant.FSerial sections of a wild‐type and a Sox7−/− embryo at 17–18 ss (E9.0), stained with endomucin (blue) to detect the endothelial lining, ERG (white) for endothelial nuclei, and SOX17 (red) for arterial nuclei. The dorsal aorta (DA) is labeled by the red arrows and white arrowheads, while cardinal vein (CV), blue arrows. Fusion between DA and CV is indicated by orange arrowheads.Data information: Scale bars = 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37551717), licensed under a CC-BY license. Not internally tested by R&D Systems.