Human Stabilin-1 Antibody (840449)

R&D Systems | Catalog # MAB3825

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 840449
View all Stabilin-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Stabilin-1
Gly2208-Val2305
Accession # Q9NY15

Specificity

Detects human Stabilin-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Stabilin-1 Antibody (840449)

Stabilin-1 antibody in Human Liver by Immunohistochemistry (IHC-P).

Stabilin‑1 in Human Liver.

Stabilin-1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Stabilin-1 Monoclonal Antibody (Catalog # MAB3825) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Stabilin-1 Antibody (840449)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Reviewed Applications

Read 1 review rated 5 using MAB3825 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Stabilin-1

Stabilin-1, also known as FEEL-1 (fasciclin, EGF-like, laminin type EGF-like, and link domain containing scavenger receptor-1) and CLEVER-1 (common lymphatic endothelial and vascular endothelial receptor-1), was originally identified as the MS-1 antigen. It is a type I transmembrane multi-domain protein with a large extracellular domain that contains 7 fasciclin domains, multiple EGF-like and laminin type EGF-like domains, and a link domain. The short cytoplasmic tail interacts with cargo adaptors that link cargo into the clathrin-coated pits (CGAs). Stabilin-1 is expressed by tissue macrophages and sinusoidal endothelial cells, which are scavenging cells. Stabilin-1 is involved in intracellular trafficking pathways and binds multiple ligands, including acetylated LDL, SPARC, AGE, and SI-CLP. An alternatively spliced, soluble, 803 amino acid (aa) Stabilin-1 that diverges after aa 746 has been identified. Within the region used as immunogen, human Stabilin-1 shares 83% and 84% amino acid sequence homology with mouse and canine Stabilin-1, respectively.

Alternate Names

CLEVER-1, FEEL-1, FELE-1, FEX1, MS-1 Antigen, STAB1, Stabilin1

Entrez Gene IDs

23166 (Human); 192187 (Mouse); 290559 (Rat)

Gene Symbol

STAB1

UniProt

Q9NY15

Additional Stabilin-1 Products

Product Documents for Human Stabilin-1 Antibody (840449)

Certificate of Analysis

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Product Specific Notices for Human Stabilin-1 Antibody (840449)

For research use only

Citations for Human Stabilin-1 Antibody (840449)

Customer Reviews for Human Stabilin-1 Antibody (840449) (1)

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  • Human Stabilin-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Skin cancer tissue
    Species: Human
    Verified Customer | Posted 12/15/2021
    Human Stabilin-1 Antibody (840449) MAB3825

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