Human TACE/ADAM17 Cytosolic Domain Antibody (136133)

R&D Systems | Catalog # MAB21291

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Intracellular Staining by Flow Cytometry, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 136133
View all TACE/ADAM17 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TACE/ADAM17
Asp695-Cys824
Accession # P78536

Specificity

Detects human TACE/ADAM17 Cytosolic Domain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human ADAM33 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TACE/ADAM17 Cytosolic Domain Antibody (136133)

Detection of Human TACE/ADAM17 antibody by Western Blot.

Detection of Human TACE/ADAM17 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TACE/ADAM17 Cytosolic Domain Monoclonal Antibody (Catalog # MAB21291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TACE/ADAM17 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TACE/ADAM17 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Cytosolic Domain Monoclonal Antibody (Catalog # MAB21291, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Western Blot Shows Human TACE/ADAM17 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human TACE/ADAM17 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TACE/ADAM17 knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TACE/ADAM17 Cytosolic Domain Monoclonal Antibody (Catalog # MAB21291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TACE/ADAM17 at approximately 140 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TACE/ADAM17 Cytosolic Domain Antibody (136133)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human TACE/ADAM17 (Catalog # 930-ADB), see our available Western blot detection antibodies

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Knockout Validated

TACE/ADAM17 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in TACE/ADAM17 knockout HeLa cell line.

Western Blot

2 µg/mL
Sample: A549 human lung carcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TACE/ADAM17

TACE (also known as ADAM17) is a type I membrane zinc protease of the ADAM family that functions as a major sheddase on the cell surface.

Long Name

TNF-alpha Converting Enzyme

Alternate Names

ADAM17, CD156b

Entrez Gene IDs

6868 (Human); 11491 (Mouse)

Gene Symbol

ADAM17

UniProt

P78536

Additional TACE/ADAM17 Products

Product Documents for Human TACE/ADAM17 Cytosolic Domain Antibody (136133)

Certificate of Analysis

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Product Specific Notices for Human TACE/ADAM17 Cytosolic Domain Antibody (136133)

For research use only

Citations for Human TACE/ADAM17 Cytosolic Domain Antibody (136133)

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Protocols

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