Human TACE/ADAM17 Ectodomain Antibody (111633)

R&D Systems | Catalog # MAB9301

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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 111633
View all TACE/ADAM17 Primary Antibodies »

Product Specifications

Immunogen

Insect ovarian cell line T. ni-derived recombinant human TACE/ADAM17
Pro18-Asn671
Accession # P78536

Specificity

Detects the ectodomain of human TACE/ADAM17 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with the ectodomain of recombinant human ADAM8, 9, 15 and recombinant mouse ADAM10 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TACE/ADAM17 Ectodomain Antibody (111633)

Detection of TACE/ADAM17 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Ectodomain Monoclonal Antibody (Catalog # MAB9301, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.
TACE/ADAM17 Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line.

TACE/ADAM17 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

TACE/ADAM17 knockout HeLa epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Monoclonal Antibody (Catalog # MAB9301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the TACE/ADAM17 knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Detection of Human TACE/ADAM17 by Flow Cytometry

Detection of Human TACE/ADAM17 by Flow Cytometry

Effects of the engineered S197P mutation on CD16a shedding in NK cells.NK92 cells transduced with empty vector (vector only), CD16a, or CD16a/S197P were treated without (Unstim.) or with PMA (100ng/ml) for 30 minutes at 37°C (A), with IL-12 and IL-18 (100ng/ml and 400ng/ml, respectively) for 24 hours at 37°C (B), or with Raji cells and rituximab for 60 min at 37°C (C). Cell surface levels of CD16a were determined by flow cytometry. Isotype-matched negative control antibody staining is indicated by a dotted line. (D) Parent NK92 cells and transduced cells expressing CD16a or CD16a/S197P were treated with Raji cells and rituximab in the presence or absence of the ADAM17 inhibitor BMS566394 (5μM) for 60 min at 37°C. Soluble CD16a levels were determined by ELISA. Each treatment condition was repeated 3 times and the data are representative of 3 independent experiments. Bar graphs show mean ± SD. Statistical significance is indicated as ***P<0.001. (E) NK92 cells expressing CD16a or CD16a/S197P were stained with the anti-ADAM17 mAbs M220, 623, 633, or an isotype-matched negative control antibody, as indicated. (F) CD56+CD45+ NK cells derived from mock-transduced iPSCs (left panel) or iPSCs expressing recombinant CD16a or CD16a/S197P (right panels) were incubated with or without K562 target cells for 4 hours at 37°C. For all histogram plots, the x-axis = Log 10 fluorescence, the y-axis = cell number, and the data are representative of at least 3 independent experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0121788), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TACE/ADAM17 Ectodomain Antibody (111633)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human TACE/ADAM17 (Catalog # 930-ADB), see our available Western blot detection antibodies

Knockout Validated

TACE/ADAM17 is specifically detected in HeLa human carcinoma parental cell line but is not detectable in TACE/ADAM17 knockout HeLa cell line.

Western Blot

1 µg/mL
Sample: Recombinant Human TACE/ADAM17 Western Blot Standard (Catalog # WBC029) under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 4.5 using MAB9301 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TACE/ADAM17

TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of Tumor Necrosis Factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.

References

  1. Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
  2. Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.

Long Name

TNF-alpha Converting Enzyme

Alternate Names

ADAM17, CD156b

Entrez Gene IDs

6868 (Human); 11491 (Mouse)

Gene Symbol

ADAM17

UniProt

P78536

Additional TACE/ADAM17 Products

Product Documents for Human TACE/ADAM17 Ectodomain Antibody (111633)

Certificate of Analysis

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Product Specific Notices for Human TACE/ADAM17 Ectodomain Antibody (111633)

For research use only

Citations for Human TACE/ADAM17 Ectodomain Antibody (111633)

Customer Reviews for Human TACE/ADAM17 Ectodomain Antibody (111633) (2)

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  • Human TACE/ADAM17 Ectodomain Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: A431 cells
    Species: Human
    Verified Customer | Posted 09/30/2021
    Human TACE/ADAM17 Ectodomain Antibody (111633) MAB9301
  • Human TACE/ADAM17 Ectodomain Antibody
    Name: Anonymous
    Application: Immunofluorescence in fresh tissue
    Sample Tested: Mesenteric artery-whole
    Species: Mouse
    Verified Customer | Posted 04/30/2019

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Protocols

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