Human TACI/TNFRSF13B Antibody

R&D Systems | Catalog # AF174

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Blockade of Receptor-ligand Interaction

Cited:

Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TACI/TNFRSF13B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B
Ser2-Thr166
Accession # Q53F36

Specificity

Detects human TACI in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TACI and recombinant human BCMA is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TACI/TNFRSF13B Antibody

TACI/TNFRSF13B antibody in Human Spleen by Immunohistochemistry (IHC-P).

TACI/TNFRSF13B in Human Spleen.

TACI/TNFRSF13B was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human TACI/TNFRSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF174) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific labeling was localized to lymphocytes in the marginal zone. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Human TACI / TNFRSF13B ELISA Standard Curve

Human TACI / TNFRSF13B ELISA Standard Curve

Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC) was serially diluted and captured by Mouse Anti-Human TACI/TNFRSF13B Monoclonal Antibody (Catalog # MAB174) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TACI/TNFRSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF174) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human TACI/TNFRSF13B Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.075-0.3 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) to immobilized Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

0.1 µg/mL
Sample: Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular
N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.

References

  1. Xia, X-Z. et al. (2000) J. Exp. Med. 192:137.
  2. von Bulow, G.U. et al. (1997) Science 278:138.
  3. Gross, J.A. et al. (2000) Nature 404:995.
  4. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  5. Yan, M. et al. (2000) Nature Immunol. 1:37.
  6. Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

Long Name

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Alternate Names

CD267, CVID, TNFRSF13B

Entrez Gene IDs

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Gene Symbol

TNFRSF13B

UniProt

Q53F36

Additional TACI/TNFRSF13B Products

Product Documents for Human TACI/TNFRSF13B Antibody

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Product Specific Notices for Human TACI/TNFRSF13B Antibody

For research use only

Citations for Human TACI/TNFRSF13B Antibody

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Protocols

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