Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 998524
View all TAP2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human TAP2
Accession # Q03519

Specificity

Detects human TAP2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TAP2 Antibody

Detection of Human TAP2 antibody by Western Blot.

Detection of Human TAP2 by Western Blot.

Western blot shows lysates of human tonsil tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TAP2 Monoclonal Antibody (Catalog # MAB2432) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TAP2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TAP2 antibody in U-251 MG and HL-60 Human Cell Line by Immunocytochemistry (ICC).

TAP2 in U-251 MG and HL-60 Human Cell Line.

TAP2 was detected in immersion fixed U-251 MG human glioblastoma cell line (left panel) and HL-60 human acute promyelocytic leukemia cell line (right panel, negative control) using Mouse Anti-Human TAP2 Monoclonal Antibody (Catalog # MAB2432) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TAP2 antibody in Human Tonsil by Immunohistochemistry (IHC-P).

TAP2 in Human Tonsil.

TAP2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human TAP2 Monoclonal Antibody (Catalog # MAB2432) at 8 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human TAP2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample:

Immersion fixed U-251 MG human glioblastoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Western Blot

2 µg/mL
Sample: Human tonsil tissue and HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB2432 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TAP2

TAP2 transports antigens from the cytoplasm to the endoplasmic reticulum, where they can bind to MHC class 1 molecules.  It does this translocation by selecting peptides based on both their length and their sequence.  The peptide selection occurs during the first step of the translocation process.  Transmembrane segments of TAP form a pore in the membrane and the peptide binding site is formed by the cytosolic component of the pore.  Inherited deficiency in the TAP transporter can lead to recurrent respiratory bacterial infections due to HLA class I deficiency (BLS1).

References

  1. Nijenjuis M., Hämmerling Journal of immunology; 1996 Dec 15;157(12):5467-5477
  2. de la Salle H, Hanau D, Fricker D, Urlacher A, Kelly A, Salamero J, Powis SH, Donato L, Bausinger H, Laforet M, (1994), Science, Jul 8; 265(5169):237-241.

Long Name

Transporter 2, ATP Binding Cassette Subfamily B Member

Alternate Names

ABC18, ABCB3, APT2, PSF2, RING11

Entrez Gene IDs

6891 (Human); 21355 (Mouse); 24812 (Rat)

Gene Symbol

TAP2

UniProt

Q03519

Additional TAP2 Products

Product Documents for Human TAP2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TAP2 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Diabetes

Customer Reviews for Human TAP2 Antibody (2)

5 out of 5
2 Customer Ratings
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  • Human TAP2 Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: breast cancer MCF7 cells and HEK293 cell lysate
    Species: Human
    Verified Customer | Posted 11/24/2022
    Western Blot: whole cell lysates from MCF-7, HCC1954 and HEK293T cells were loaded with 50 ug/lane. 10% SDS-PAGE. TAP2 Antibody (MAB2432) was used for primary antibody: 1:1000, 4℃, overnight
    Human TAP2 Antibody MAB2432
  • Human TAP2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Glial cells
    Species: Human
    Verified Customer | Posted 06/22/2022
    Human TAP2 Antibody MAB2432

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Protocols

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