Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 622782
View all TCF7L1/TCF3 Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human TCF7L1
Lys429-Ser581
Accession # Q9HCS4

Specificity

Detects human TCF7L1 in direct ELISAs. In direct ELISAs, 5-10% cross-reactivity with recombinant human (rh) TCF-4 (aa 102-516) and rhTCF-12 is observed. No cross-reactivity with rhTCF7L1 (aa 151-320), rhTCF7L2, rhTCF-1, -2, -3, -4 (aa 241-365), rhLEF-1, or recombinant mouse TCF-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TCF7L1 Antibody

TCF7L1 antibody in Human Brain by Immunohistochemistry (IHC-P).

TCF7L1 in Human Brain.

TCF7L1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human TCF7L1 Monoclonal Antibody (Catalog # MAB61161) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human TCF7L1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TCF7L1/TCF3

Transcription factor 7-like 1 (TCF7L1; also HMG box transcription factor 3, TCF-3) is a 63 kDa member of the TCF/LEF family of proteins.  Human TCF7L1 is 588 amino acids (aa) in length and contains one HMG box DNA-binding domain (aa 346-414).  Human TCF7L1 shares 95% aa sequence identity with mouse TCF7L1.  TCF7L1 is expressed in hair follicles and skin keratinocytes and at lower levels in stomach epithelium.  Functionally, it participates in the Wnt signaling pathway.  TCF7L1 binds to DNA and acts as a repressor in the absence of CTNNB1 and as an activator in its presence.  TCF7L1 is also necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules, and the development of the barrier function of the epidermis.

Long Name

Transcription Factor 7-like 1

Alternate Names

TCF3

Entrez Gene IDs

83439 (Human); 21415 (Mouse); 312451 (Rat)

Gene Symbol

TCF7L1

UniProt

Q9HCS4 (Human)

Additional TCF7L1/TCF3 Products

Product Documents for Human TCF7L1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TCF7L1 Antibody

For research use only

Citations for Human TCF7L1 Antibody

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Protocols

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