Home / TGF-beta 1 / Human TGF-beta 1 PE-conjugated Antibody

Human TGF-beta 1 PE-conjugated Antibody

Catalog #: IC240P
8 Citations Datasheet / COA / SDS

Discontinued Product

IC240P has been discontinued.
View all TGF-beta 1 products.
Detection of TGF‑ beta 1 in PC‑3 human prostate cancer cell line by Flow Cytometry.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TGF-beta 1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than 2% cross‑reactivity with recombinant human (rh) TGF‑ beta 3 and recombinant amphibian TGF‑ beta 5 and no cross‑reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 9016
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant TGF‑ beta 1 and latent TGF‑ beta 1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of TGF-beta 1 antibody in PC-3 human prostate cancer cell line antibody by Flow Cytometry. View Larger

Detection of TGF‑ beta 1 in PC‑3 human prostate cancer cell line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TGF-beta 1 PE-conjugated Monoclonal Antibody (Catalog # IC240P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta 1

TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

Long Name
Transforming Growth Factor beta 1
Entrez Gene IDs
7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names
CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Product Datasheets

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Citations for Human TGF-beta 1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. The effect of hepatocyte growth factor on secretory functions in human eosinophils
    Cytokine, 2016-08-20;88(0):45-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Mesenchymal stromal cells induce epithelial-to-mesenchymal transition in human colorectal cancer cells through the expression of surface-bound TGF-beta.
    Authors: Mele V, Muraro M, Calabrese D, Pfaff D, Amatruda N, Amicarella F, Kvinlaug B, Bocelli-Tyndall C, Martin I, Resink T, Heberer M, Oertli D, Terracciano L, Spagnoli G, Iezzi G
    Int J Cancer, 2014-01-30;134(11):2583-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Changes of peripheral TGF-beta1 depend on monocytes-derived macrophages in Huntington disease.
    Authors: Di Pardo A, Alberti S, Maglione V, Amico E, Cortes E, Elifani F, Battaglia G, Busceti C, Nicoletti F, Vonsattel J, Squitieri F
    Mol Brain, 2013-12-13;6(0):55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Myeloid dendritic cells (DCs) of mice susceptible to paracoccidioidomycosis suppress T cell responses whereas myeloid and plasmacytoid DCs from resistant mice induce effector and regulatory T cells.
    Authors: Pina A, de Araujo E, Felonato M, Loures F, Feriotti C, Bernardino S, Barbuto J, Calich V
    Infect Immun, 2013-01-22;81(4):1064-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Identification of IDO-positive and IDO-negative human dendritic cells after activation by various proinflammatory stimuli.
    Authors: Von Bubnoff D, Scheler M, Wilms H, Fimmers R, Bieber T
    J. Immunol., 2011-05-04;186(12):6701-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Expression of the T cell receptor alpha/beta on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
    Authors: Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
    PLoS ONE, 2011-01-11;6(1):e15997.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation.
    Authors: Cedeno-Laurent F, Barthel SR, Opperman MJ, Lee DM, Clark RA, Dimitroff CJ
    J. Immunol., 2010-09-15;185(8):4659-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
    Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
    J. Immunol., 2008-04-01;180(7):5109-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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