Human Thimet Oligopeptidase/THOP1 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF3439R Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3439R-100UG

Save 15% on Select RUO Reagents. See Details.

All Thimet Oligopeptidase/THOP1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Thimet Oligopeptidase/THOP1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Oligopeptidase/THOP1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Neurolysin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Oligopeptidase/THOP1
Lys2-Cys689
Accession # P52888

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Thimet Oligopeptidase/THOP1

Thimet Oligopeptidase/THOP1, also known as endopeptidase EC 3.4.24.15 (EP24.15), is a zinc peptidase of the M3 family that also includes neurolysin/EC 3.4.24.16 and mitochondrial intermediate peptidase (1). Widely expressed by mammalian tissues and reported to present in different subcellular locations, THOP1 is primarily a cytoplasmic enzyme. It is capable of hydrolyzing a number of bioactive peptides and peptides released by the proteosome, limiting antigenic presentation by MHC class I molecules (1‑3). THOP1 also interacts with angiotensin II type I receptor and bradykinin B2 receptor (4). The optimal activity of the purified THOP1 may or may not require the presence of a reducing agent, depending upon the source of the enzyme and the purification method used.

Entrez Gene IDs

7064 (Human); 50492 (Mouse); 64517 (Rat)

Alternate Names

EC 3.4.24; EC 3.4.24.15; Endopeptidase 24.15; EP24.15; MEPD_HUMAN; MP78; thimet oligopeptidase 1; thimet oligopeptidase; THOP1; TOP