Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Antibody Array Development, ELISA Detection

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all TIMP-2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human TIMP‑2 (R&D Systems, Catalog # 971-TM)
Cys27-Pro220
Accession # P16035

Specificity

Detects human TIMP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) TIMP‑1, recombinant rat TIMP‑1, recombinant mouse TIMP‑1, and rhTIMP-3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TIMP-2 Antibody [Biotin]

Human TIMP-2 ELISA Standard Curve

Human TIMP-2 ELISA Standard Curve

Recombinant Human TIMP‑2 (Catalog # 971-TM) was serially diluted and captured by Mouse Anti-Human TIMP‑2 Monoclonal Antibody (Catalog # MAB9711) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TIMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human TIMP-2 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TIMP‑2 Western Blot Standard (Catalog # 971-TM)

Human TIMP-2 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human TIMP‑2 Antibody (Catalog # MAB9711)
  • Standard: Recombinant Human TIMP-2 Protein, CF (Catalog # 971-TM)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF971 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIMP-2

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.

Long Name

Tissue Inhibitors of Metalloproteinases 2

Alternate Names

TIMP2

Entrez Gene IDs

7077 (Human); 21858 (Mouse); 29543 (Rat)

Gene Symbol

TIMP2

UniProt

P16035

Additional TIMP-2 Products

Product Documents for Human TIMP-2 Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TIMP-2 Antibody [Biotin]

For research use only

Citations for Human TIMP-2 Antibody [Biotin]

Customer Reviews for Human TIMP-2 Antibody [Biotin] (1)

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  • Human TIMP-2 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/16/2017
    BAF971 was used with MAB9711 to develop an ELISA to measure TIMP-2 in human plasma samples. This antibody was used as the detection molecule.
    Human TIMP-2 Antibody [Biotin] BAF971

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