Human TROP-2 Antibody (77220)

R&D Systems | Catalog # MAB650

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Bioassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 77220
View all TROP-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TROP-2
His27-Thr274
Accession # P09758

Specificity

Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) VCAM‑1 or rhICAM‑1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TROP-2 Antibody (77220)

Detection of TROP-2 antibody in PC-3 Human Cell Line antibody by Flow Cytometry.

Detection of TROP‑2 in PC-3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TROP-2 Monoclonal Antibody (Catalog # MAB650, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
Detection of TROP‑2 in A431 Human Cell Line by Flow Cytometry.

Detection of TROP‑2 in A431 Human Cell Line by Flow Cytometry.

Flow cytometry histograms of A431 wild-type cells (left graph) and A431-TROP2 Knock-out cells (right graph). Both cell lines were incubated with FAB650P before the analysis. Image from a verified customer review. The customer used the PE-conjugated version of MAB650, which is listed under FAB650P.
Detection of TROP-2 by Flow Cytometry

Detection of TROP-2 by Flow Cytometry

Human blastocyst-like structures form analogs of pre-implantation lineages.a. Flow cytometry analysis plot of cells isolated from blastocyst-like structures and stained for lineage-specific surface markers PDGFRa (PrE) and TROP2 (TE). The gates were used to sort analogs of EPI (double negative), TE (TROP2high) and PrE (PDGFR alpha high) to subsequently process for single cell RNA sequencing. Note that the gates did not exclude any cells. This analysis was performed to correlate RNA measures, while ensuring a representation of all cell types. b. UMAPs of the transcriptome of single cells isolated from blastocyst-like structures and displaying the expression levels of genes specific for each of the three blastocyst lineages (TE - Trophectoderm, EPI - Epiblast, and PrE- Primitive endoderm). c-g. UMAPs of single cells isolated from both blastocyst-like structures and from embryos ranging from E3 to E19. c. Coloration of cells originating from In Vitro Fertilization (IVF) embryos isolated on day 3 (E3) to day 7 (E7). This period comprises only pre-implantation stage embryos. d. Coloration of cells originating from IVF embryos isolated on day 6 (E6) to day 12 (E12). These blastocysts (E6) were cultured in vitro. Note that this annotation reflects the number of days in culture rather than the developmental stages. e. Coloration of cells originating from gastrulation-stage embryo isolated on day 17 (E17) to 19 (E19). f. The expression levels of genes specific for each of the three blastocyst lineages (EPI, TE, and PrE). g. Coloration of cells displaying their unsupervised cluster affiliation. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34856602), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TROP-2 Antibody (77220)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: PC‑3 human prostate cancer cell line

Western Blot

1 µg/mL
Sample: Recombinant Human TROP‑2 Fc Chimera (Catalog # 650-T2)

Reviewed Applications

Read 3 reviews rated 3.3 using MAB650 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TROP-2

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

References

  1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
  2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
  3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
  4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
  5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

Long Name

Tumor-associated Calcium Signal Transducer 2

Alternate Names

GA733-1, gp50, T16, TACSTD2, TROP2

Entrez Gene IDs

4070 (Human); 56753 (Mouse)

Gene Symbol

TACSTD2

UniProt

P09758

Additional TROP-2 Products

Product Documents for Human TROP-2 Antibody (77220)

Certificate of Analysis

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Product Specific Notices for Human TROP-2 Antibody (77220)

For research use only

Citations for Human TROP-2 Antibody (77220)

Customer Reviews for Human TROP-2 Antibody (77220) (3)

3.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
33%
3 Stars
0%
2 Stars
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1 Stars
33%

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Customer Images

Showing  1 - 3 的 3 reviews Showing All
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  • Human TROP-2 Antibody
    Name: Anonymous
    Application: Protein: Protein Interaction
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 08/24/2021
    Ligand: Anti-Trop2 Analyte: human Trop2-his 1:1 fitting with KD 10nM
    Human TROP-2 Antibody (77220) MAB650
  • Human TROP-2 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Breast cancer cell line MCF7
    Species: Human
    Verified Customer | Posted 12/23/2020
    Detection of TROP2 on human breast cancer cell line MCF7. MCF7 cells were treated with 67 nM of the human TROP2 antibody (catalog # MAB650) or mouse IgG2a isotype control, followed by a secondary antibody goat a-mouse IgG Fc APC. Isotype control (RED), human TROP2 antibody (Blue).
    Human TROP-2 Antibody (77220) MAB650
  • Human TROP-2 Antibody
    Name: Elizabeth Pongo
    Application: Binding by Octet
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 06/08/2020
    Human TROP-2 Antibody (77220) MAB650

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