Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Hamster, Monkey, Primate - Macaca mulatta (Rhesus Macaque), Transgenic Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all VASA Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VASA
Met1-Tyr145
Accession # Q9NQI0

Specificity

Detects human VASA in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human VASA Antibody

Detection of Human VASA antibody by Western Blot.

Detection of Human VASA by Western Blot.

Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VASA antibody in Human Testis by Immunohistochemistry (IHC-P).

VASA in Human Testis.

VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Mouse VASA by Immunohistochemistry

Detection of Mouse VASA by Immunohistochemistry

Immuno-staining on control and PMSG treated ovarian sections.A-C: Immunostaining with anti-PCNA antibody. Note minimal PCNA staining in control OSE. Increased staining is observed after PMSG treatment in both the OSE cells and the oocytes of PF (B-&C). Inset B’ is magnified image of the PF located in the OSE. At places, granulosa cells were also positive for PCNA (arrowhead). D-F: Immunostaining with anti-OCT-4 antibody in control (D) and 7D PMSG treated (E&F) ovarian sections. OCT-4 is localized in the ooplasm in control, while in 7D PMSD treated, positive staining was observed in ooplasm as well as in the nucleus of few oocytes (arrowhead) in PFs. G: Immunostaining with anti-human VASA antibody that cross-reacts with mouse MVH. MVH is distinctly localized in the ooplasm of PF and at places was also observed in a ‘germ cell nest’ (G’, arrow). At places some oocytes of primordial follicles in cohorts appeared connected without intervening granulosa cell (arrowhead) H-J: SCP-3 is localized in PF oocytes present in close vicinity of multilayer OSE. Bar: 20μm. Image collected and cropped by CiteAb from the following publication (https://ovarianresearch.biomedcentral.com/articles/10.1186/1757-2215-5-…), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human VASA Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

1 µg/mL
Sample: Human testis tissue

Reviewed Applications

Read 6 reviews rated 5 using AF2030 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VASA

The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is therefore a highly specific marker of germ cells (1-3).

References

  1. Zeeman, A.M. et al. (2002) Lab Invest. 82:159.
  2. Raz, E. (2000) Genome Biol. 1:REVIEWS1017.
  3. Castrillon, D. et al. (2001) Proc. Natl. Acad. Sci. USA 97:9585.

Long Name

DEAD [Asp-Glu-Ala-Asp] Box Polypeptide 41

Alternate Names

DDX4

Entrez Gene IDs

54514 (Human); 13206 (Mouse); 310090 (Rat)

Gene Symbol

DDX4

UniProt

Q9NQI0

Additional VASA Products

Product Documents for Human VASA Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VASA Antibody

For research use only

Related Research Areas

Primordial and Mature Germ Cells

Citations for Human VASA Antibody

Customer Reviews for Human VASA Antibody (6)

5 out of 5
6 Customer Ratings
5 Stars
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4 Stars
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Customer Images

Showing  1 - 5 的 6 reviews Showing All
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  • Human VASA IF
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Testis tissue
    Species: Human
    Verified Customer | Posted 02/20/2025
    Vasa signaling specific label human germ cell.
    Sections were treated with xylene for deparaffinization and ethanol for rehydration. Antigen retrieval was achieved by microwaving in 10 mM citrate-based buffer at pH 6.0. Sections were washed by PBS with 0.05% Tween 20, followed by incubation with VASA antibodies overnight at 4°C and secondary antibodies at room temperature for 1 h.
    Human VASA Antibody AF2030
  • Human VASA Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Ovary tissue
    Species: Mouse
    Verified Customer | Posted 11/15/2024
    Worked on mouse P14 ovary magenta (laminin), green (vasa), working concentration 1:100, IHC-P
    Human VASA Antibody AF2030
  • Human VASA Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Ovary tissue
    Species: Mouse
    Verified Customer | Posted 11/08/2024
    The antibody was tested on a paraffin-embedded section of a mouse P14 ovary, with successful staining observed at a dilution of 1:200. Green (VASA) Magenta (Laminin)
    Human VASA Antibody AF2030
  • Human VASA Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Ovary tissue
    Species: Mouse
    Verified Customer | Posted 11/16/2023
    Whole mount of Mouse Ovary (E16.5). Dilution: 1:300.
    Human VASA Antibody AF2030
  • Human VASA Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Testis tissue
    Species: Mouse
    Verified Customer | Posted 11/11/2019
    DDX4/VASA germ cell marker. IF1:500
    Human VASA Antibody AF2030
  • Human VASA Affinity Purified Polyclonal Ab
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Testis tissue
    Species: Mouse
    Verified Customer | Posted 04/13/2016
    Postnatal day 6 mouse testes were fixed in 4% paraformaldehyde. Tissue was embedded in O.C.T. and 5 micron sections cut. Sections were permeabilized with PBS containing 0.1% Triton X-100, then blocked for 30 min in 3% BSA in PBS containing 0.1% Triton X-100. Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (AF2030) was diluted in blocking buffer to 1.25 µg/ml and applied to sections for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100, then incubated with secondary antibody: Donkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 555 conjugate (Thermo Fisher A-21432) diluted to 1:500 for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100 and mounted.
    Human VASA Antibody AF2030

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