Human WISP‑1/CCN4 Biotinylated Antibody

R&D Systems | Catalog # BAF1627

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all WISP-1/CCN4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human WISP-1
Thr23-Asn367
Accession # O95388

Specificity

Detects human WISP-1 in Western blots. In this format, approximately 50% cross‑reactivity with recombinant mouse (rm) WISP‑1 is observed and less than 1% cross‑reactivity with rhNOV and rhCTGF is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human WISP‑1/CCN4 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human WISP‑1/CCN4 (Catalog # 1627-WS)

Human WISP-1/CCN4 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human WISP‑1/CCN4 Antibody (Catalog # MAB16271)
  • Standard: Recombinant Human WISP-1/CCN4 Protein, CF (Catalog # 1627-WS)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: WISP-1/CCN4

Human WISP-1 (Wnt-induced secreted protein-1; also CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1‑5). It is synthesized as a 367 aa precursor that contains a series of structural homology modules. Following a 22 amino acid (aa) signal sequence, there is a 68 aa IGFBP-like domain (aa 53‑120), a 57 aa von Willebrand factor type C (VWC) module (aa 126‑182), a 40 aa TSP type I domain (aa 220‑259) and a 75 aa, C-terminal cysteine knot motif (aa 273‑347). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). At least five alternative splice forms are known for WISP-1. One is 30 kDa in size, 258 aa in length, and shows a substitution of a His for aa 95‑182. This removes the VWC domain (2, 6). A second isoform is 155 aa in length and shows a frameshift at Arg 117 with a unique 38 aa C‑terminal extension. A third is 195 aa in length and shows a 31 aa substitution for the first 203 aa of the full length precursor (6). This retains the VWC and cysteine knot domains. A fourth shows a 43 aa substitution for aa 117‑367 for a total length of 163 aa. This effectively removes everything but the IGFBP-like domain (7). The last splice form contains a deletion of aa 25‑269 for a total length of 122 aa. Thus, only the signal sequence and cysteine knot motifs are retained (8). This leaves only the IGFBP-like domain (9). Full-length mature human WISP-1 is 85% aa identical to both mouse and rat WISP-1. WISP-1 is expressed by osteoblasts and may contribute to fracture healing by promoting bone cell formation (10, 11).

References

  1. Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
  2. Tanada, S. et al. (2001) Oncogene 20:5525.
  3. Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
  4. Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
  5. Brigstock, D.R. (2003) J. Endocrinol. 178:169.
  6. Li, Z. (2005) GenBank Accession # AAP43925.
  7. Li, Z. (2005) GenBank Accession # AAP43926.
  8. Li, Z. (2005) GenBank Accession # AAP43924.
  9. Cervello, M. et al. (2004) Ann. N.Y. Acad. Sci. 1028:432.
  10. French, D.M. et al. (2004), Am. J. Pathol. 165:855.
  11. Parisi, M.S. et al. (2006) Bone 38:671.

Long Name

Wnt-1 Induced Secreted Protein 1

Alternate Names

CCN4, WISP1

Entrez Gene IDs

8840 (Human); 22402 (Mouse); 102125044 (Cynomolgus Monkey)

Gene Symbol

CCN4

UniProt

O95388

Additional WISP-1/CCN4 Products

Product Documents for Human WISP‑1/CCN4 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human WISP‑1/CCN4 Biotinylated Antibody

For research use only

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