ID3 Antibody (2B11) - Azide and BSA Free

Novus Biologicals | Catalog # NB500-112

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B11

Format

Azide and BSA Free
View all ID3 Primary Antibodies »

Product Specifications

Immunogen

The full-length (amino acids 1-119) mouse ID3 gene expressed in E. coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Nuclear

Specificity

NB500-112 reacts with human and mouse Id3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ID3 Antibody (2B11) - Azide and BSA Free

Western Blot: ID3 Antibody (2B11) [NB500-112]

Western Blot: ID3 Antibody (2B11) [NB500-112]

Western Blot: ID3 Antibody (2B11) [NB500-112] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Id3 antibody [2B11] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ID3 Antibody (2B11) [NB500-112]

Immunocytochemistry/ Immunofluorescence: ID3 Antibody (2B11) [NB500-112]

Immunocytochemistry/Immunofluorescence: ID3 Antibody (2B11) [NB500-112] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Id3 protein stained by Id3 antibody [2B11] diluted at 1:100. Blue: Hoechst 33342 staining.Scale bar = 10 um.

Applications for ID3 Antibody (2B11) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1-5 ug/ml

Western Blot

1-5 ug/ml
Application Notes
Use in FLOW reported in scientific literature (PMID: 21081927).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ID3

Id3, a 14kDa protein that is a member of the Id family of helix-loop-helix proteins, is believed to be a dominant negative regulator of bHLH proteins involved in cell differentiation and implicated in the control of cell growth, cellular differentiation, apoptosis and carcinogenesis.

Alternate Names

BHLHB25, bHLHb251R21, Class B basic helix-loop-helix protein 25, DNA-binding protein inhibitor ID-3, HEIR1, HEIR-1, Helix-loop-helix protein HEIR-1, ID-like protein inhibitor HLH 1R21, Inhibitor of DNA binding 3, inhibitor of DNA binding 3, dominant negative helix-loop-helix protein

Entrez Gene IDs

3399 (Human)

Gene Symbol

ID3

Additional ID3 Products

Product Documents for ID3 Antibody (2B11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ID3 Antibody (2B11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ID3 Antibody (2B11) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ID3 Antibody (2B11) - Azide and BSA Free

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  • Q: Can this antibody (2B11) be applied for mouse IHC-P?

    A:

    For our ID3 antibody NB500-112, we have not yet tested this antibody in IHC. It has only been validated for detection of the human and mouse protein in western blot, ICC, and IP. If you did want to try this product in IHC with mouse samples, this would also be covered by our Innovator's Reward Program.

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