IDH2 Antibody

Novus Biologicals | Catalog # NBP1-85784

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all IDH2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: REPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFD

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25643707).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IDH2 Antibody

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784]

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784]

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining in human heart muscle and testis tissues using NBP1-85784 antibody. Corresponding IDH2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784]

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784]

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human heart muscle shows high expression.
Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784]

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784]

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human testis shows weak to moderate granular cytoplasmic positivity in a small subset of cells in seminiferous ducts.
Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784]

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784]

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human colorectal cancer shows strong granular cytoplasmic positivity in tumor cells.
Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784]

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784]

Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human prostate shows moderate to granular cytoplasmic positivity in smooth muscle cells.
IDH2 Antibody Immunohistochemistry: IDH2 Antibody [IDH2 Antibody]

Immunohistochemistry: IDH2 Antibody [IDH2 Antibody]

Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.

Applications for IDH2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
IHC reported in scientific literature (PMID: 25643707). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IDH2

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Alternate Names

D2HGA2, EC 1.1.1.42, ICD-M, IDH, IDHM, IDP, IDPM, isocitrate dehydrogenase [NADP], mitochondrial, isocitrate dehydrogenase 2 (NADP+), mitochondrial, mNADP-IDH, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

Entrez Gene IDs

3418 (Human); 269951 (Mouse); 361596 (Rat)

Gene Symbol

IDH2

UniProt

P48735 (Human)

Additional IDH2 Products

Product Documents for IDH2 Antibody

Certificate of Analysis

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Product Specific Notices for IDH2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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