IFI27 Antibody

Name: IFI27 Antibody
Brand: Novus Biologicals
SKU: NB300-709
Price: 599 USD
Availability: InStock

Novus Biologicals | Catalog # NB300-709

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Product Documents

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Synthetic peptide: multiple antigenic peptide (8 branch) corresponding to amino acids 2-13 of Human IFI27.

Epitope

N-epitope

Specificity

IFI27

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for IFI27 Antibody

Application

Recommended Usage

ELISA

1:10000-1:1000

Immunoprecipitation

1:150

Western Blot

1:1500

Application Notes

Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IFI27

Treatment of breast carcinoma MCF7cells with estradiol led to induction of a new 27 kDa protein, the cDNA was later cloned and identified as p27. This highly hydrophobic protein of 122 amino acids has 33% over all sequence similarity to the product of the 6-16 gene (1), that is induced by alfa/beta type interferons. It has been shown that p27is transcriptionaly induced by interferon alfa in various human cell lines. The induction induced by interferon alfa is independent of the presence of estradiol receptors on the cells. High levels of p27 mRNA, detected by Northern blotting, was found in 50% of the primary breast carcinoma cells, suggesting p27 can be a diagnostic marker for breast carcinoma detection (2). Examination of p27 expression by in situ hybridization in p27 over expressing tumors suggest that p27 gene is localized in cancer cells and some times also in fibroblastic cells of tumor stroma. IFI27 mRNA expression is highly up-regulated in lesional psoriatic epidermis and in non-lesional keratinocytes. It was also expressed in lichen planus, chronic eczema, cutaneous squamous cell cancers, and during normal wound repair when IFI27 was found in the proliferating subpopulation of keratinocytes (3). P27 Further studies are now necessary to elucidate the cause of p27 gene overexpression in breast carcinoma and in particular to determine whether it corresponds to chromosomal rearrangements in the 14q32 region and/or to induction by interferons of the alpha/beta type.

Alternate Names

FAM14D, Interferon alpha-induced 11.5 kDa protein, interferon alpha-inducible protein 27, mitochondrial, interferon, alpha-inducible protein 27,2310061N23Rik, Interferon-stimulated gene 12a protein, ISG12, ISG12(a), ISG12A, P27

Entrez Gene IDs

3429 (Human)

Gene Symbol

IFI27

Additional IFI27 Products

Product Documents for IFI27 Antibody

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Certificate of Analysis

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Product Specific Notices for IFI27 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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