Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Rat IgA

Specificity

By immunoelectrophoresis and ELISA this reacts specifically with rat IgA. This may cross react with IgA from other species

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Goat anti-Rat IgA Heavy Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:2000

Immunohistochemistry

1:200 - 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Western Blot

1:1000-1:30000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Phosphate Buffered Saline (PBS)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgA Heavy Chain

Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA.

Additional IgA Heavy Chain Products

Product Documents for Goat anti-Rat IgA Heavy Chain Secondary Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Goat anti-Rat IgA Heavy Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Goat anti-Rat IgA Heavy Chain Secondary Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Goat anti-Rat IgA Heavy Chain Secondary Antibody

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  • Q: I would like to know if Rat IgA Antibody (NB7107) can be applied in IF for tissue sections? What's the difference between IF/ICC and IF?

    A: Our rat IgA antibody with catalogue number NB7107 is covered by our 100% guarantee for immunohistochemistry with paraffin-embedded rat samples, irrespective of whether you use a fluorescent detection method or DAB staining. ICC/IF stands for immunocytochemistry / immunofluorescence, and refers to the staining of cells. IHC stands for immunohistochemistry and refers to the staining of tissues. IHC-P indicates that the antibody has been validated with paraffin-embedded samples. NB7107 has not yet been tested for IHC with frozen tissue, which would be listed as IHC-Fr.

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