Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

HRP

Antibody Source

Polyclonal Rabbit IgG
View all IgA Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Human IgA alpha heavy chain

Specificity

Specificity was confirmed by ELISA minimal cross reactivity against other Human heavy or light chain isotypes.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgA and Human Serum

Scientific Data Images for Rabbit anti-Human IgA Heavy Chain Secondary Antibody [HRP]

ELISA: Rabbit anti-Human IgA Heavy Chain Secondary Antibody [HRP] [NBP1-73613] - Absorbance values for antigen-specific IgA in human plasma samples. Image from verified customer review.
ELISA: Rabbit anti-Human IgA Heavy Chain Secondary Antibody [HRP] [NBP1-73613]

Applications for Rabbit anti-Human IgA Heavy Chain Secondary Antibody [HRP]

Application
Recommended Usage

ELISA

1:56000

Immunohistochemistry

1:500 - 1:2500

Western Blot

1:1000 - 1:5000
Application Notes
This product has been tested by ELISA and is designed for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Reviewed Applications

Read 1 review rated 4 using NBP1-73613 in the following applications:

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Product Documents for Rabbit anti-Human IgA Heavy Chain Secondary Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rabbit anti-Human IgA Heavy Chain Secondary Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Rabbit anti-Human IgA Heavy Chain Secondary Antibody [HRP] (1)

4 out of 5
1 Customer Rating
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Customer Images

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  • Rabbit anti-Human IgA Heavy Chain Secondary Antibody [HRP]
    Name: Mona Agrawal
    Application: ELISA
    Sample Tested: Blood plasma
    Species: Human
    Verified Customer | Posted 11/23/2022
    Absorbance values for antigen-specific IgA in human plasma samples.
    Rabbit anti-Human IgA Heavy Chain Secondary Antibody [HRP] NBP1-73613

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Protocols

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FAQs

No product specific FAQs exist for this product.

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