Goat F(ab')2 anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody [Biotin] (Pre-adsorbed)

Novus Biologicals | Catalog # NB120-6005

Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed
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Product Specifications

Immunogen

Mouse IgG IgA and IgM whole molecule

Specificity

No reaction was observed against Human Serum proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room

This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum

Applications

Application
Recommended Usage

ELISA

1:1200000

Immunohistochemistry

1:2000 - 1:20000

Western Blot

1:5000 - 1:20000
Application Notes
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Format

Pre-adsorbed

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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