Key Product Details

Species Reactivity

Chicken

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG
View all IgG F(ab')2 Secondary Antibodies »

Product Specifications

Immunogen

Chicken IgG F(ab')2 fragment

Specificity

No reaction was observed against Chicken IgG F(c).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab')2 and Chicken Serum

Scientific Data Images for Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [Biotin]

Western Blot: Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [Biotin] [NBP1-73508] - Western Blot of Rabbit anti-Chicken IgG F(ab')2 Secondary antibody [Biotin]. Lane M: 3 ul Molecular Ladder. Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment. Lane 3: Chicken IgG F(ab) Fragment. All samples were reduced. Load: 50 ng per lane. Block: 30 min at RT. Primary Antibody: Anti-Chicken IgG F(ab')2 (RABBIT) Antibody 1:5,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.
Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [Biotin]

Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [Biotin]

Western Blot of Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [Biotin]

Applications for Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [Biotin]

Application
Recommended Usage

ELISA

1:20000 - 1:100000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:2000 - 1:10000
Application Notes
This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:34,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Product Documents for Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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