Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, ELISA

Label

HRP

Antibody Source

Polyclonal Rabbit IgG
View all IgG F(ab')2 Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG F(ab')2 fragment

Specificity

No reaction was observed against Mouse IgG F(c).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum

Scientific Data Images for Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [HRP]

Western Blot: Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [HRP] [NBP1-73640] - WB shows detection of mouse anti-beta Actin monoclonal antibody (Catalog# NBP1-47423) using a HRP conjugated rabbit anti-mouse IgG, F(ab')2 secondary antibody (Catalog# NBP1-73640). Image from verified customer review.

Applications for Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [HRP]

Application
Recommended Usage

ELISA

1:10000 - 1:50000

Immunohistochemistry

1:500 - 1:2500

Western Blot

1:1000 - 1:10000
Application Notes
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:12,000 is suggested for this product.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Reviewed Applications

Read 1 review rated 5 using NBP1-73640 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Product Documents for Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [HRP] (1)

5 out of 5
1 Customer Rating
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4 Stars
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: human cancer cell lysate
    Species: Human
    Verified Customer | Posted 10/08/2015
    Mouse IgG, Fab2 Antibody (NBP1-73640)
    Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [HRP] NBP1-73640

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Protocols

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FAQs for Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [HRP]

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  • Q: I have couple of questions for you about your NBP1-73640 antibody. What exactly is this antibody? Is this antibody a Fab2 fragment only? And the data sheet says that Mouse IgG F(ab')2 fragment was used as an immunogen. When using Mouse IgG F(ab')2 fragment as an immunogen what does the antibody recognize versus when using Mouse IgG F(c) fragment as an immunogen? Are there some differences? I would like to have anti-Mouse IgG antibody that is IgG F(ab')2 fragment conjugated with HRP.

    A: NBP1-73640 is affinity purified whole rabbit IgG which binds to the F(ab)2 part of mouse IgG. IgG normally is composed of two longer heavy chains with two shorter light chains in a Y shape. The arms of the Y are composed of both heavy and light chains and are the F(ab)2 or Fab fragments. The stem of the Y is the F(C) portion formed from the lower part of the heavy chains.

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