Key Product Details

Species Reactivity

Bovine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

HRP

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Rabbit anti-Bovine IgG Fc fragment Secondary Antibody was produced by repeated immunization with bovine IgG F(c) fragment in rabbit.

Specificity

No reaction was observed against Bovine IgG F(ab).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(c) and Bovine Serum

Scientific Data Images for Rabbit anti-Bovine IgG Fc Secondary Antibody [HRP]

ELISA: Rabbit anti-Bovine IgG Fc Secondary Antibody [HRP] [NBP1-73581] - ELISA Results of Rabbit anti-Bovine IgG Fc Secondary antibody [HRP] tested against purified Bovine IgG Fc HRP. Each well was coated in duplicate with 1.0 ug of Bovine IgG Fc. The working dilution is 1:15,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.

Applications for Rabbit anti-Bovine IgG Fc Secondary Antibody [HRP]

Application
Recommended Usage

ELISA

1:10000 - 1:50000

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:2500

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

1:1000 - 1:10000
Application Notes
This product has been tested by ELISA. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product. NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Polyethylene Glycol (PEG-8000)

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Product Documents for Rabbit anti-Bovine IgG Fc Secondary Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rabbit anti-Bovine IgG Fc Secondary Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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