Key Product Details

Species Reactivity

Armenian Hamster

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IgG (H+L) Secondary Antibodies »

Product Specifications

Immunogen

Armenian Hamster IgG whole molecule

Specificity

Reactivity with Golden Syrian Hamster IgG is greatly reduced.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum

Scientific Data Images for Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [Biotin]

Western Blot: Goat anti-Armenian Hamster IgG Secondary Antibody [Biotin] [NB120-5744] - Lane 1: Armenian Hamster IgG. Lane 2: none. Load: 50 ug per lane. Primary antibody: Armenian Hamster IgG Biotin conjugated antibody at 1:1,000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Human IgG.
Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [Biotin]

Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [Biotin]

Western Blot of Biotin conjugated Goat anti-Armenian Hamster IgG antibody. Lane 1: Armenian Hamster IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: Armenian Hamster IgG Biotin conjugated antibody at 1:1,000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Human IgG. Other band(s): none.

Applications for Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [Biotin]

Application
Recommended Usage

ELISA

1:20000 - 1:100000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:2000 - 1:10000
Application Notes
This product has been tested by ELISA and western blot and is designed for Western Blotting, ELISA and Immunohistochemistry. Biotin conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for Goat anti-Armenian Hamster IgG (H+L) Secondary Antibody [Biotin]

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  • Q: If I use NBP2-59616 with NB120-5744 as secondary antibody, where is the binding place of secondary one (Fc or Fab)?

    A: NB120-5744 was raised against the Armenian Hamster IgG whole molecule as the immunogen. As a polyclonal antibody, this product will therefore target varying epitopes along the entire immunogen sequence. We therefore cannot state that it will specifically bind to either the Fab or Fc region of its target.

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