IgG2 Antibody (RM118) [Biotin]

Novus Biologicals | Catalog # NBP2-62076B

Recombinant Monoclonal Antibody.
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM118 expressed in HEK293
View all IgG2 Primary Antibodies »

Product Specifications

Immunogen

Human IgG2

Specificity

This antibody reacts to the heavy chain of human IgG2. This antibody does not cross react to any other IgG subclasses (IgG1, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, or IgE. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG2 Antibody (RM118) [Biotin]

IgG2 Antibody (RM118) [Biotin]

ELISA: IgG2 Antibody (RM118) [Biotin] [NBP2-62076B] -

ELISA: IgG2 Antibody (RM118) [Biotin] [NBP2-62076B] - Sandwich ELISA using NBP2-62076B as the capture antibody, and biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.
IgG2 Antibody (RM118) [Biotin]

ELISA: IgG2 Antibody (RM118) [Biotin] [NBP2-62076B] -

ELISA: IgG2 Antibody (RM118) [Biotin] [NBP2-62076B] - Sandwich ELISA using NBP2-62076B as the capture antibody, and biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.
IgG2 Antibody (RM118) [Biotin]

ELISA: IgG2 Antibody (RM118) [Biotin] [NBP2-62076B] -

ELISA: IgG2 Antibody (RM118) [Biotin] [NBP2-62076B] - ELISA of human immunoglobulins shows NBP2-62076B only reacts to human IgG2. No cross reactivity with Human IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with different immunoglobulins. A serial dilution of NBP2-62076B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG2 Antibody (RM118) [Biotin]

ELISA: IgG2 Antibody (RM118) - Azide and BSA Free [NBP2-62076B] -

ELISA: IgG2 Antibody (RM118) - Azide and BSA Free [NBP2-62076B] - A titer ELISA using RM118. The plate was coated with different amounts of human IgG2. A serial dilution of RM118 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG2 Antibody (RM118) [Biotin]

Application
Recommended Usage

Immunohistochemistry

0.5 - 2 ug/mL

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG2

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2

Alternate Names

Constant region of heavy chain of IgG2, immunoglobulin gamma 2 (Gm marker), immunoglobulin heavy constant gamma 2 (Gm marker)

Gene Symbol

IGHG2

Additional IgG2 Products

Product Documents for IgG2 Antibody (RM118) [Biotin]

Certificate of Analysis

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Product Specific Notices for IgG2 Antibody (RM118) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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