Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Immunofluorescence

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Human IgG2
View all IgG2 Isotype Controls »

Product Specifications

Clonality

Polyclonal

Host

Human

Isotype

IgG2

Description

This Human IgG2 immunoglobulin is useful as an isotype control for Human IgG2 antibody.

Applications for Human IgG2 Isotype Control [Alexa Fluor® 488]

Application
Recommended Usage

Flow (Cell Surface)

1:10 - 1:1000

Flow (Intracellular)

1:10 - 1:1000

Flow Cytometry

1:10-1:1000

Immunofluorescence

1:10 - 1:500
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS, 50% glycerol.

Preservative

No Preservative

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: IgG2

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2

Alternate Names

Constant region of heavy chain of IgG2, immunoglobulin gamma 2 (Gm marker), immunoglobulin heavy constant gamma 2 (Gm marker)

Gene Symbol

IGHG2

Additional IgG2 Products

Product Documents for Human IgG2 Isotype Control [Alexa Fluor® 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Human IgG2 Isotype Control [Alexa Fluor® 488]

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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