Rabbit IgM Isotype Control

Novus Biologicals | Catalog # NBP1-96968

Novus Biologicals
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Key Product Details

Species Reactivity

Rabbit

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation, SDS-Page

Label

Unconjugated

Antibody Source

Rabbit IgM

Format

Purified
View all IgM Isotype Controls »

Product Specifications

Specificity

No reaction was observed against anti-Rabbit IgG F(c). Some light chain cross-reactivity will occur with anti-Rabbit IgG.

Host

Rabbit

Isotype

IgM

Description

This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and anti-Rabbit IgM (u chain specific)

Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Rabbit IgM Isotype Control

Western Blot: Rabbit IgM Isotype Control [NBP1-96968]

Western Blot: Rabbit IgM Isotype Control [NBP1-96968]

Western Blot: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
SDS-PAGE: Rabbit IgM Isotype Control [NBP1-96968]

SDS-PAGE: Rabbit IgM Isotype Control [NBP1-96968]

SDS-Page: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.

Applications for Rabbit IgM Isotype Control

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

SDS-Page

1:100 - 1:2000

Western Blot

1:100 - 1:2000
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Format

Purified

Preservative

0.1% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.

Background: IgM

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for Rabbit IgM Isotype Control

Certificate of Analysis

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Product Specific Notices for Rabbit IgM Isotype Control

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Protocols

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