Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all IL-12 p70 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human IL-12 p70/IL-12A (Accession#: P29459; Met 1-Ser 219)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for IL-12 p70/IL-12A Antibody - Azide and BSA Free

Western Blot: IL-12 p70/IL-12A Antibody [NBP2-98678]

Western Blot: IL-12 p70/IL-12A Antibody [NBP2-98678]

Western Blot: IL-12 p70/IL-12A Antibody [NBP2-98678] - Lane A: MOLT4 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:38 kDa (We are unsure as to the identity of these extra bands.)
Immunohistochemistry-Paraffin: IL-12 p70/IL-12A Antibody [NBP2-98678]

Immunohistochemistry-Paraffin: IL-12 p70/IL-12A Antibody [NBP2-98678]

Immunohistochemistry-Paraffin: IL-12 p70/IL-12A Antibody [NBP2-98678] - Human breast carcinoma with rabbit polyclonal antibody (1:1000).

Applications for IL-12 p70/IL-12A Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry

1:500-1:2000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-12 p70

Interleukin-12, or IL-12, also known as natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine that affects both natural killer (NK) cells and T lymphocytes. IL-12 is a heterodimer of subunit IL-12A p35 and subunit IL-12B p40 that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. It is involved in mediating many aspects of antiviral immunity. In addition to stimulating T helper cell development, IL-12 is a potent inducer of interferon-gamma (IFN-gamma) production by T cells and NK cells. Increased IL-12 production is a consequence of EBV, CMV, LCMV or HSV infection. While it is unclear what separate effects can be attributed to each IL-12 subunit, evidence suggests IL-12A p35 is the biologically active component responsible for signal transduction via the IL-12 receptor.

Long Name

Interleukin 12 p70

Alternate Names

IL12 p70

Gene Symbol

IL12A

Additional IL-12 p70 Products

Product Documents for IL-12 p70/IL-12A Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IL-12 p70/IL-12A Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-12 p70/IL-12A Antibody - Azide and BSA Free

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    • Q: Does the human IL-12 polyclonal antibody (Catalog # AB-219-NA) recognize IL-12 p70?
      A: In Western blots, this antibody recognizes the p70 heterodimer as well as the p40 and p35 subunits.
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