Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44395

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL6937

Format

Azide and BSA Free
View all Integrin alpha 3/CD49c Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P26006, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free (NBP2-76515) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free

Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Analysis in human lung and liver tissues using NBP3-44395 antibody. Corresponding Integrin alpha 3/CD49c RNA-seq data are presented for the same tissues.
Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Staining of human colon shows strong membranous positivity in glandular cells.
Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Staining of human lung shows strong membranous positivity in pneumocytes.
Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Staining of human liver shows no positivity in hepatocytes as expected.
Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free Western Blot: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Western Blot: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Analysis in human cell line U-87 MG.
Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free Western Blot: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Western Blot: Integrin alpha 3/CD49c Antibody [NBP3-44395]

Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Integrin alpha 3/CD49c antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Applications for Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 - 1:250

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 3/CD49c

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 3 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that interacts with many extracellular-matrix proteins. The alpha 3 beta 1 integrin is known variously as: very late (activation) antigen 3 (VLA3), very common antigen 2 (VCA2), extracellular matrix receptor 1 (ECMR1), and galactoprotein b3 (GAPB3).

Alternate Names

CD49c, ITGA3

Gene Symbol

ITGA3

Additional Integrin alpha 3/CD49c Products

Product Documents for Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Integrin alpha 3/CD49c Antibody (CL6937) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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