Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Janelia Fluor 669

Antibody Source

Monoclonal Mouse IgG1 Clone # 212701
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Integrin  beta 2/CD18
Gln23-Asn700
Accession # AAA59490

Specificity

Detects human Integrin beta 2/CD18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin  beta 1 or recombinant mouse Integrin  alpha 5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Integrin beta 2/CD18 Antibody (212701) [Janelia Fluor® 669]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Integrin beta 2/CD18

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Alternate Names

CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta

Gene Symbol

ITGB2

Additional Integrin beta 2/CD18 Products

Product Documents for Integrin beta 2/CD18 Antibody (212701) [Janelia Fluor® 669]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Integrin beta 2/CD18 Antibody (212701) [Janelia Fluor® 669]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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