Integrin beta 2/CD18 Antibody - BSA Free

Name: Integrin beta 2/CD18 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-88128
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-88128

Submit a review

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all Integrin beta 2/CD18 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CTKFKVSSCRECIESGPGCTWCQKLNFTGPGDPDSIRCDTRPQLLMRGCAADDIMDPTSLAETQEDHNGGQKQLSPQKVTLYLRPGQAAAFNVTFRRAKGYPIDLYYLMDLSYSML

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Integrin beta 2/CD18 Antibody - BSA Free (NBP1-88128) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Integrin beta 2/CD18 Antibody - BSA Free

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128] - Staining of human gastrointestinal, lymph node, skeletal muscle and tonsil using Anti-Integrin beta 2/CD18 antibody NBP1-88128 (A) shows similar protein distribution across tissues to independent antibody NBP1-88127 (B).

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128] - Staining of human lymph node shows strong membranous positivity in germinal center cells.

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody - BSA Free [NBP1-88128]

Analysis in human lymph node and skeletal muscle tissues using HPA008877 antibody. Corresponding ITGB2 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128] - Staining of human colon shows strong membranous positivity in lymphoid cells.

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128]

Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88128] - Staining of human tonsil shows strong membranous positivity in germinal center cells.

Western Blot: Integrin beta 2/CD18 Antibody - BSA Free [NBP1-88128]

Analysis using Anti-ITGB2 antibody NBP1-88128 (A) shows similar pattern to independent antibody NBP1-88127 (B).

View 4 more images

Applications for Integrin beta 2/CD18 Antibody - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 2/CD18

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Alternate Names

CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta

Gene Symbol

ITGB2

Additional Integrin beta 2/CD18 Products

Product Documents for Integrin beta 2/CD18 Antibody - BSA Free

Download Product Datasheets

Download SDS

Product Specific Notices for Integrin beta 2/CD18 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.