JPH1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83076

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all JPH1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KADAADQAALAARQECDIARAVARELSPDFYQPGPDYVKQRFQEGVDAKENPEEKVPEKPPTPKESPHFYRKGTTPPRSPEASPKHSHSPASSPKPLKKQNPSSGARLNQDKRSVADEQVTAIVNK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JPH1 Antibody - BSA Free

Western Blot: JPH1 Antibody [NBP1-83076]

Western Blot: JPH1 Antibody [NBP1-83076]

Western Blot: JPH1 Antibody [NBP1-83076] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: JPH1 Antibody [NBP1-83076]

Immunocytochemistry/ Immunofluorescence: JPH1 Antibody [NBP1-83076]

Immunocytochemistry/Immunofluorescence: JPH1 Antibody [NBP1-83076] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: JPH1 Antibody [NBP1-83076]

Immunohistochemistry-Paraffin: JPH1 Antibody [NBP1-83076]

Immunohistochemistry-Paraffin: JPH1 Antibody [NBP1-83076] - Staining of human prostate shows low expression as expected.
Immunohistochemistry-Paraffin: JPH1 Antibody [NBP1-83076]

Immunohistochemistry-Paraffin: JPH1 Antibody [NBP1-83076]

Immunohistochemistry-Paraffin: JPH1 Antibody [NBP1-83076] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: JPH1 Antibody [NBP1-83076]

Immunohistochemistry-Paraffin: JPH1 Antibody [NBP1-83076]

Immunohistochemistry-Paraffin: JPH1 Antibody [NBP1-83076] - Staining in human skeletal muscle and prostate tissues using anti-JPH1 antibody. Corresponding JPH1 RNA-seq data are presented for the same tissues.

Applications for JPH1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JPH1

JPH1 is a gene which codes for a protein that contributes to the formation of skeletal muscles, as well as a membrane complex which links the plasma membrane to the endoplasmic/sarcoplasmic reticulum as well as serves as a structural base for communication between the cell surface and intracellular calcium channels and is 661 amino acids long, weighing approximately 72 kDa. Current studies are being done on diseases and disorders related to this gene such as pneumonia, malaria, and neuronitis. JPH1 has also been shown to have interactions with SMAD3, TP63, TP73, JPH2, and JPH3.

Alternate Names

DKFZp762L0313, JP-1junctophilin type1, JP1junctophilin-1, junctophilin 1, Junctophilin type 1, mitsugumin72

Gene Symbol

JPH1

Additional JPH1 Products

Product Documents for JPH1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for JPH1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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