KAP1 Antibody (4E6) - Azide and BSA Free

Novus Biologicals | Catalog # H00010155-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4E6

Format

Azide and BSA Free
View all KAP1 Primary Antibodies »

Product Specifications

Immunogen

TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIER

Specificity

TRIM28 (4E6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for KAP1 Antibody (4E6) - Azide and BSA Free

Western Blot: KAP1 Antibody (4E6) [H00010155-M01]

Western Blot: KAP1 Antibody (4E6) [H00010155-M01]

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6. Analysis of TRIM28 expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: KAP1 Antibody (4E6) [H00010155-M01]

Immunocytochemistry/ Immunofluorescence: KAP1 Antibody (4E6) [H00010155-M01]

Immunocytochemistry/Immunofluorescence: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of monoclonal antibody to TRIM28 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: KAP1 Antibody (4E6) [H00010155-M01]

Immunohistochemistry-Paraffin: KAP1 Antibody (4E6) [H00010155-M01]

Immunohistochemistry-Paraffin: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Western Blot: KAP1 Antibody (4E6) [H00010155-M01]

Western Blot: KAP1 Antibody (4E6) [H00010155-M01]

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi ( Cat # H00010155-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody (M01) clone 4E6 (Cat # H00010155-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: KAP1 Antibody (4E6) [H00010155-M01]

Western Blot: KAP1 Antibody (4E6) [H00010155-M01]

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6. Analysis of TRIM28 expression in PC-12.
Western Blot: KAP1 Antibody (4E6) [H00010155-M01]

Western Blot: KAP1 Antibody (4E6) [H00010155-M01]

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6 Analysis of TRIM28 expression in Hela S3 NE.
Western Blot: KAP1 Antibody (4E6) [H00010155-M01]

Western Blot: KAP1 Antibody (4E6) [H00010155-M01]

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody (M01), clone 4E6.Lane 1: TRIM28 transfected lysate(88.5 KDa).Lane 2: Non-transfected lysate.

Applications for KAP1 Antibody (4E6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation, and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: KAP1

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Long Name

KRAB-associated Protein 1

Alternate Names

KRIP-1, RNF96, TF1B, TIF1B, TRIM28

Entrez Gene IDs

10155 (Human)

Gene Symbol

TRIM28

OMIM

601742 (Human)

UniProt

AAH04978 (Human)

Additional KAP1 Products

Product Documents for KAP1 Antibody (4E6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for KAP1 Antibody (4E6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KAP1 Antibody (4E6) - Azide and BSA Free

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Protocols

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FAQs

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