KAT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87835

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all KAT1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KFKINKQHARRVYEILRLLVTDMSDAEQYRSYRLDIKRRLISPYKKKQRDLAKMRKCLRPEELTNQMNQIEISMQHEQLEESFQELVEDYRRVIERLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KAT1 Antibody - BSA Free

Western Blot: KAT1 Antibody [NBP1-87835]

Western Blot: KAT1 Antibody [NBP1-87835]

Western Blot: KAT1 Antibody [NBP1-87835] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-HAT1 antibody. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: KAT1 Antibody [NBP1-87835]

Immunocytochemistry/ Immunofluorescence: KAT1 Antibody [NBP1-87835]

Immunocytochemistry/Immunofluorescence: KAT1 Antibody [NBP1-87835] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835]

Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835]

Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835] - Staining in human lymph node and pancreas tissues using anti-HAT1 antibody. Corresponding HAT1 RNA-seq data are presented for the same tissues.
Western Blot: KAT1 Antibody [NBP1-87835]

Western Blot: KAT1 Antibody [NBP1-87835]

Western Blot: KAT1 Antibody [NBP1-87835] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835]

Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835]

Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835] - Staining of human lymph node shows high expression.
Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835]

Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835]

Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835] - Staining of human pancreas shows low expression as expected.

Applications for KAT1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KAT1

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.

Alternate Names

EC 2.3.1.48, histone acetyltransferase 1, histone acetyltransferase type B catalytic subunit, KAT1

Gene Symbol

HAT1

Additional KAT1 Products

Product Documents for KAT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for KAT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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