Laminin beta 3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89956

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Laminin beta 3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLVTSMTKQLGDFWTRMEELRHQARQQGAEAVQAQQLAEGASEQALSAQEGFERIKQKYAELKDRLGQSSMLGEQGARIQSVKTEAEELFGETMEMMDRMKDMELELLRGSQAIMLRSADLTGLEKRVEQ

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23159910).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Laminin beta 3 Antibody - BSA Free

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP1-89956]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP1-89956]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP1-89956] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Laminin beta 3 Antibody - BSA Free Western Blot: Laminin beta 3 Antibody - BSA Free [NBP1-89956]

Western Blot: Laminin beta 3 Antibody - BSA Free [NBP1-89956]

Analysis in human cell lines A-431 and MCF-7 using Anti-LAMB3 antibody. Corresponding LAMB3 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Applications for Laminin beta 3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. WB reactivity reported in (PMID: 23159910).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin beta 3

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.

Alternate Names

BM600-125kDa, Epiligrin subunit bata, FLJ99565, Kalinin B1 chain, Kalinin subunit beta, kalinin-140kDa, LAM5, Laminin 5, Laminin B1k chain, laminin S B3 chain, laminin subunit beta-3, laminin, beta 3, laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)), Laminin-5 subunit beta, LAMNB1, Nicein subunit beta, nicein-125kDa

Gene Symbol

LAMB3

Additional Laminin beta 3 Products

Product Documents for Laminin beta 3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Laminin beta 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Laminin beta 3 Antibody - BSA Free

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Protocols

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FAQs

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