![Western Blot: LARS Antibody [NBP2-32209]](https://resources.rndsystems.com/images/products/LARS-Antibody-Western-Blot-NBP2-32209-img0001.jpg "Western Blot: LARS Antibody [NBP2-32209]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Orangutan (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 250 to 300 of human Leucyl-tRNA Synthetase using the numbering given in entry NP_064502.9 (GeneID 51520).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LARS Antibody - BSA Free
Western Blot: LARS Antibody [NBP2-32209]
Western Blot: LARS Antibody [NBP2-32209] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-LARS antibody NBP2-32209 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry-Paraffin: LARS Antibody [NBP2-32209]](https://resources.rndsystems.com/images/products/LARS-Antibody-Immunohistochemistry-NBP2-32209-img0002.jpg "Immunohistochemistry-Paraffin: LARS Antibody [NBP2-32209]")
Immunohistochemistry-Paraffin: LARS Antibody [NBP2-32209]
Immunohistochemistry-Paraffin: LARS Antibody [NBP2-32209] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARNT used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).Applications for LARS Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Western Blot
1:1000 - 1:5000
Application Notes
Immunoprecipitation Not recommended. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: LARS
Alternate Names
cytoplasmic leucyl-tRNA synthetase, FLJ21788, hr025Cl, HSPC192, KIAA1352, LARS1, leucine translase, leucine tRNA ligase 1, cytoplasmic, leucine-tRNA ligase, leucine--tRNA ligase, leucyl-tRNA synthetase, leucyl-tRNA synthetase, cytoplasmic, LEURS, LEUS, LRS, PIG44
Gene Symbol
LARS
UniProt
Additional LARS Products
Product Documents for LARS Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LARS Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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