LIN-28A Antibody (2S1I10)
Novus Biologicals | Catalog # NBP3-16843
Recombinant Monoclonal Antibody
![Western Blot: LIN-28A Antibody (2S1I10) [NBP3-16843]](https://beta-resources.rndsystems.com/images/products/LIN-28A-Antibody-2S1I10-Western-Blot-NBP3-16843-img0004.jpg "Western Blot: LIN-28A Antibody (2S1I10) [NBP3-16843]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2S1I10 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LIN-28A (Q9H9Z2). MGSVSNQQFAGGCAKAAEEAPEEAPEDAARAADEPQLLHGAGICKWFNVRMGFGFLSMTARAGVALDPPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit LIN-28A Antibody (2S1I10) (NBP3-16843) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for LIN-28A Antibody (2S1I10)
Western Blot: LIN-28A Antibody (2S1I10) [NBP3-16843]
Western Blot: LIN-28A Antibody (2S1I10) [NBP3-16843] - Western blot analysis of extracts of various cell lines, using LIN-28A antibody (NBP3-16843) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.![Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]](https://beta-resources.rndsystems.com/images/products/LIN-28A-Antibody-2S1I10-Immunohistochemistry-NBP3-16843-img0003.jpg "Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]")
Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]
Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843] - Immunofluorescence analysis of mouse testis using LIN-28A Rabbit mAb (NBP3-16843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]](https://beta-resources.rndsystems.com/images/products/LIN-28A-Antibody-2S1I10-Immunohistochemistry-NBP3-16843-img0001.jpg "Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]")
Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]
Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843] - Immunofluorescence analysis of rat testis using LIN-28A Rabbit mAb (NBP3-16843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]](https://beta-resources.rndsystems.com/images/products/LIN-28A-Antibody-2S1I10-Immunohistochemistry-NBP3-16843-img0002.jpg "Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]")
Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]
Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843] - Immunofluorescence analysis of human ovary using LIN-28A Rabbit mAb (NBP3-16843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for LIN-28A Antibody (2S1I10)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:2000 - 1:20000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: LIN-28A
Long Name
RNA-binding Protein LIN-28
Alternate Names
CSDD1, LIN28, LIN28A, Tex17, ZCCHC1
Gene Symbol
LIN28A
Additional LIN-28A Products
Product Documents for LIN-28A Antibody (2S1I10)
Product Specific Notices for LIN-28A Antibody (2S1I10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
